IKB beta/NFKBIB Protein, Human (His)

IKB beta/NFKBIB protein effectively inhibits NF-kappa-B and prevents NF-kappa-B activation by forming a cytoplasmic complex. After cell stimulation, the non-phosphorylated form is resynthesized, binds to NF-kappa-B, aids nuclear transport, and protects NF-kappa-B from NFKBIA-dependent inactivation. IKB beta/NFKBIB Protein, Human (His) is the recombinant human-derived IKB beta/NFKBIB protein, expressed by E. coli , with N-6*His labeled tag.

IKB beta/NFKBIB protein functions as a potent inhibitor of NF-kappa-B by forming a complex with it and sequestering it in the cytoplasm. Upon cell stimulation, the unphosphorylated form is resynthesized, enabling its binding to NF-kappa-B and facilitating its transport to the nucleus. This protects NF-kappa-B from further NFKBIA-dependent inactivation. Additionally, its association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevents phosphorylation, rendering it more resistant to degradation and explaining its slower turnover. IKB beta/NFKBIB protein exhibits interactions with THRB, RELA, and REL, contributing to its regulatory role. Moreover, it interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2, adding further complexity to its modulatory functions.

Human

E. coli

N-His

Q15653 (M1-V356)

4793  [NCBI]

MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

Approximately 45 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.