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IKB beta/NFKBIB Protein, Human (His)

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IKB beta/NFKBIB protein effectively inhibits NF-kappa-B and prevents NF-kappa-B activation by forming a cytoplasmic complex. After cell stimulation, the non-phosphorylated form is resynthesized, binds to NF-kappa-B, aids nuclear transport, and protects NF-kappa-B from NFKBIA-dependent inactivation. IKB beta/NFKBIB Protein, Human (His) is the recombinant human-derived IKB beta/NFKBIB protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

IKB beta/NFKBIB protein effectively inhibits NF-kappa-B and prevents NF-kappa-B activation by forming a cytoplasmic complex. After cell stimulation, the non-phosphorylated form is resynthesized, binds to NF-kappa-B, aids nuclear transport, and protects NF-kappa-B from NFKBIA-dependent inactivation. IKB beta/NFKBIB Protein, Human (His) is the recombinant human-derived IKB beta/NFKBIB protein, expressed by E. coli , with N-6*His labeled tag.

Background

IKB beta/NFKBIB protein functions as a potent inhibitor of NF-kappa-B by forming a complex with it and sequestering it in the cytoplasm. Upon cell stimulation, the unphosphorylated form is resynthesized, enabling its binding to NF-kappa-B and facilitating its transport to the nucleus. This protects NF-kappa-B from further NFKBIA-dependent inactivation. Additionally, its association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevents phosphorylation, rendering it more resistant to degradation and explaining its slower turnover. IKB beta/NFKBIB protein exhibits interactions with THRB, RELA, and REL, contributing to its regulatory role. Moreover, it interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2, adding further complexity to its modulatory functions.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His
  • Accession

    Q15653-1/NP_002494.2 (M1-V356)

  • Gene ID
    4793  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • NFKBIB (M1-V356)
      Accession # Q15653-1/NP_002494.2
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    NFKBIB; TR-Interacting Protein 9; NFKB Inhibitor Beta; I-Kappa-B-Beta; TRIP9; NF-Kappa-BIB; IKBB; IkappaBbeta; NF-Kappa-B Inhibitor Beta; IkB-Beta; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Beta; TRIP-9; Thyroid Recepto

  • AA Sequence

    MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

  • Predicted Molecular Mass

    37.8 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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