IL-23R Protein, Human (HEK293, mFc)
Based on 1 Customer Validation
The IL-23R protein is critical in immune responses by binding to IL12RB1 to form the interleukin 23 receptor. It binds to IL23 and activates T cells, NK cells and potentially macrophages/myeloid cells through Jak-Stat signaling. IL-23R Protein, Human (HEK293, mFc) is the recombinant human-derived IL-23R protein, expressed by HEK293 , with C-mFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The IL-23R protein is critical in immune responses by binding to IL12RB1 to form the interleukin 23 receptor. It binds to IL23 and activates T cells, NK cells and potentially macrophages/myeloid cells through Jak-Stat signaling. IL-23R Protein, Human (HEK293, mFc) is the recombinant human-derived IL-23R protein, expressed by HEK293 , with C-mFc labeled tag.
Background
The IL-23R protein plays a critical role in immune response as it forms the interleukin-23 receptor by associating with IL12RB1. It binds to IL23 and triggers the activation of T-cells, NK cells, and potentially certain macrophage/myeloid cells through the Jak-Stat signaling cascade. IL23 is involved in both innate and adaptive immunity, and it likely contributes to the acute response to infection in peripheral tissues. Moreover, IL23 is implicated in autoimmune inflammatory diseases and has been shown to be important in tumorigenesis. When IL12RB1 is present, IL-23R forms a heterodimer, which acts as the IL-23 receptor. It interacts with JAK2 and, in the presence of IL23, activates STAT3.
Verified Bioactivity
1.Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.33 μg/mL determined by ELISA.
2.Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.
3. Immobilized Biotinylated Human IL-23 alpha&IL 12 beta, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 37.0ng/ml determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-mFc
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Accession
Q5VWK5-1 (G24-G355)
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Molecular Construction
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N-term
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IL-23R (G24-G355)
Accession # Q5VWK5-1 -
mFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IL23R; IL-23 Receptor; Interleukin 23 Receptor; IL23RL1-THE1A; Interleukin-23 Receptor; PSORS7; IL-23R
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AA Sequence
GITNINCSGHIWVEPATIFKMGMNISIYCQAAIKNCQPRKLHFYKNGIKERFQITRINKTTARLWYKNFLEPHASMYCTAECPKHFQETLICGKDISSGYPPDIPDEVTCVIYEYSGNMTCTWNAGKLTYIDTKYVVHVKSLETEEEQQYLTSSYINISTDSLQGGKKYLVWVQAANALGMEESKQLQIHLDDIVIPSAAVISRAETINATVPKTIIYWDSQTTIEKVSCEMRYKATTNQTWNVKEFDTNFTYVQQSEFYLEPNIKYVFQVRCQETGKRYWQPWSSLFFHKTPETVPQVTSKAFQHDTWNSGLTVASISTGHLTSDNRGDIG
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Predicted Molecular Mass
63.7 kDa
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Molecular Weight
Approximately 80-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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