IL-2R alpha/CD25 Protein, Cynomolgus (HEK293)
Based on 1 Customer Validation
IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions. IL-2R alpha/CD25 Protein, Cynomolgus (HEK293) is a recombinant cynomolgus IL-2R alpha protein and is expressed in HEK293 cells. It consists of 213 amino acids (M1-R213).
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions[1][2]. IL-2R alpha/CD25 Protein, Cynomolgus (HEK293) is a recombinant cynomolgus IL-2R alpha protein and is expressed in HEK293 cells. It consists of 213 amino acids (M1-R213).
Background
IL-2R alpha (CD25) is a type I membrane protein. IL-2R alpha is expressed in peripheral activated T and B cells, triple-negative thymocytes, and bone marrow pre-B cells. In high tumor regulatory T (Treg) cells, IL-2R alpha is highly expressed and is a potential target for Treg deletion. The expression of IL-2R alpha is undetectable on resting T cells[1][2][3].
The sequence of amino acids in IL-2R alpha from cynomolgus monkey shows 91.91% similarity with human IL-2R alpha.
IL-2R alpha is an essential component of high-affinity IL-2 receptors and has no signal-transducing activity per se. IL-2R alpha functions through enhancing binding of IL-2 to its receptor complex and acts as a positive feedback regulator. IL-2 is a principal growth factor for T lymphocytes and plays an important role in T cell immune response. IL-2R alpha transcription is regulated by three positive regulatory regions (PRRs): PRRI, PRRII and PRRIII. PRRIII is an IL-2 response element[1][2].
IL-2R alpha regulates T cell growth, augments lymphocyte activation and proliferation. IL-2R alpha is involved in preventing type 1 diabetes and cancers[1][2][4].
Verified Bioactivity
Immobilized CD25/IL2RA Protein, Cynomolgus, Recombinant at 2 μg/ml (100 μl/well) can bind biotinylated IL2 Protein, Human, Recombinant(ECD, hFc Tag). The EC50 for this effect is 15-90 ng/mL.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag Tag Free
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Accession
H6WS54 (E22-R213)
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Molecular Construction
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N-term
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IL-2Rα (E22-R213)
Accession # H6WS54 -
C-term
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Protein Length
Partial
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Synonyms
IL2RA; TAC Antigen; Prev. IDDM10; Insulin-Dependent Diabetes Mellitus 10; Prev. IL2R; CD25 Antigen; Interleukin-2 Receptor Subunit Alpha; IL-2-RA; CD25; IL2-RA; Interleukin 2 Receptor, Alpha; IMD41; IL-2 Receptor Subunit Alpha; TCGFR; IL-2R Subunit Alpha;
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AA Sequence
ELCDDDPPKITHATFKAVAYKEGTMLNCECKRGFRRIKSGSPYMLCTGNSSHSSWDNQCQCTSSAARNTTKQVTPQPEEQKERKTTEMQSQMQLADQVSLPGHCREPPPWENEATERIYHFVVGQTVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGETEPSQFPGEEEPQASPDGLPESETSR
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Predicted Molecular Mass
22.4 kDa
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Molecular Weight
Approximately 38-42 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
References
[1]. H.Asao. Interleukin-2. Reference Module in Biomedical Sciences. 2014. ISBN 9780128012383.
[2]. Kim HP, et al. The basis for IL-2-induced IL-2 receptor alpha chain gene regulation: importance of two widely separated IL-2 response elements. Immunity. 2001 Jul;15(1):159-72. [Content Brief]
[3]. Bell CJ, et al. Sustained in vivo signaling by long-lived IL-2 induces prolonged increases of regulatory T cells. J Autoimmun. 2015 Jan;56:66-80. [Content Brief]
[4]. Chistiakov DA, et al. The crucial role of IL-2/IL-2RA-mediated immune regulation in the pathogenesis of type 1 diabetes, an evidence coming from genetic and animal model studies. Immunol Lett. 2008 Jun 15;118(1):1-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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