Integrin alpha 5 beta 1 Protein, Human (HEK293, His)
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P08648 (F42-Y995)&P05556-1 (Q21-D728)
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Gene ID3678&3688
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Molecular Construction
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N-term
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ITGA5 (F42-Y995)
Accession # P08648 -
His
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C-term
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N-term
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P05556-1 ITGB1 (Q21-D728)
Accession # P05556-1 -
C-term
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Synonyms
ITGA5; Integrin Alpha-F; Prev. FNRA; CDNA FLJ50382, Moderately Similar To Integrin Alpha-5; CD49e; Very Late Activation Protein 5, Alpha Subunit; Integrin, Alpha 5 (Fibronectin Receptor, Alpha Polypeptide); Fibronectin Receptor, Alpha Subunit; Fibronectin
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Predicted Molecular Mass
110.7 kDa (ITGA5) & 83.7 kDa (ITGB1)
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Molecular Weight
Approximately 130-150 kDa (ITGA5) & 100-120 kDa (ITGB1), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Heterodimer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)