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JAM-A/CD321 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The JAM-A/CD321 protein is critical for the formation of epithelial tight junctions, is present in early junction development, and recruits PARD3. Binding of the PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thereby impeding tight junction assembly. JAM-A/CD321 Protein, Human (HEK293, His) is the recombinant human-derived JAM-A/CD321 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for JAM-A/CD321 Protein, Human (HEK293, His)
  • JAM-A/CD321 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The JAM-A/CD321 protein is critical for the formation of epithelial tight junctions, is present in early junction development, and recruits PARD3. Binding of the PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thereby impeding tight junction assembly. JAM-A/CD321 Protein, Human (HEK293, His) is the recombinant human-derived JAM-A/CD321 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The JAM-A/CD321 protein appears to be involved in the formation of tight junctions in epithelial cells. It is present in the early stages of cell junction development and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction between PARD3 and JAM1, thus hindering tight junction assembly. The protein also plays a role in regulating monocyte transmigration, which is crucial for maintaining the integrity of the epithelial barrier. It acts as a ligand for integrin alpha-L/beta-2, facilitating the transmigration of memory T-cells and neutrophils. Additionally, it is involved in platelet activation. In terms of microbial infection, JAM-A/CD321 acts as a receptor for Mammalian reovirus sigma-1.

Verified Bioactivity

Immobilized Human JAM-A at 1 μg/mL (100 μL/well) can bind Anti-JAM-A antibody. The ED50 for this effect is 3.4 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9Y624-1 (S28-V238)

  • Gene ID
    50848  [NCBI]
  • Molecular Construction
    • N-term
    • JAM-A (S28-V238)
      Accession # Q9Y624-1
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    F11R; JAM-A; Prev. JAM1; JAMA; Junctional Adhesion Molecule 1; Platelet Adhesion Molecule 1; PAM-1; Platelet F11 Receptor; JCAM; CD321 Antigen; Junctional Adhesion Molecule A; JAM; JAM-1; KAT; F11 Receptor; CD321

  • AA Sequence

    SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

  • Predicted Molecular Mass

    23.9 kDa

  • Molecular Weight

    Approximately 28-33 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 100 mM Glycine, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immobilized Human JAM-A at 1 μg/mL (100 μL/well) can bind Anti-JAM-A antibody. The ED50 for this effect is 3.4 ng/mL.

  • JAM-A/CD321 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for JAM-A/CD321 Protein, Human (HEK293, His)
  • JAM-A/CD321 Protein, Human (HEK293, His) Product Image