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Kininogen-1 Protein, Human (626a.a, HEK293, His)

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Kininogen-1 protein undergoes alternative splicing, producing high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for coagulation, assembly of the kallikrein-kinin system, and release of the antimicrobial bradykinin. Kininogen-1 Protein, Human (626a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Kininogen-1 Protein, Human (626a.a, HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Kininogen-1 protein undergoes alternative splicing, producing high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for coagulation, assembly of the kallikrein-kinin system, and release of the antimicrobial bradykinin. Kininogen-1 Protein, Human (626a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Kininogen-1 Protein undergoes alternative splicing to yield two distinct proteins—high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK plays a crucial role in blood coagulation and the assembly of the kallikrein-kinin system. Additionally, HMWK releases bradykinin, a peptide with various physiological effects and antimicrobial properties against bacteria and fungi. In contrast, LMWK is not implicated in blood coagulation. Notably, during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, a reduction or depletion of angiotensin converting enzyme 2 (ACE2) leads to an elevation in des-Arg(9)-bradykinin levels, a bioactive bradykinin metabolite associated with lung injury and inflammation. The gene exhibits restricted expression primarily in the liver (RPKM 432.8).

Verified Bioactivity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC and the IC50 value is < 7 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    NP_001095886.1 (Q19-S644)

  • Gene ID
    3827  [NCBI]
  • Molecular Construction
    • N-term
    • Kininogen-1 (Q19-S644)
      Accession # NP_001095886.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    KNG1; Fitzgerald Factor; Prev. BDK; Bradykinin; Prev. KNG; CDNA FLJ56836, Highly Similar To Kininogen-1; Prev. HK; High-Molecular-Weight Kininogen; HMWK; Kininogen 1, Isoform CRA_b; Alpha-2-Thiol Proteinase Inhibitor; KNG1 Protein; BK; Kininogen; Williams

  • AA Sequence

    QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTVSPPHTSMAPAQDEERDSGKEQGHTRRHDWGHEKQRKHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNGKHNGWKTEHLASSSEDSTTPSAQTQEKTEGPTPIPSLAKPGVTVTFSDFQDSDLIATMMPPISPAPIQSDDDWIPDIQIDPNGLSFNPISDFPDTTSPKCPGRPWKSVSEINPTTQMKESYYFDLTDGLS

  • Predicted Molecular Mass

    71.3 kDa

  • Molecular Weight

    Approximately 100-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris, 100 mM NaCl, pH 7.5, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Kininogen-1 Protein, Human (626a.a, HEK293, His) Product Image