Kininogen-1 Protein, Human (626a.a, HEK293, His)

Kininogen-1 protein undergoes alternative splicing, producing high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for coagulation, assembly of the kallikrein-kinin system, and release of the antimicrobial bradykinin. Kininogen-1 Protein, Human (626a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

Kininogen-1 Protein undergoes alternative splicing to yield two distinct proteins—high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK plays a crucial role in blood coagulation and the assembly of the kallikrein-kinin system. Additionally, HMWK releases bradykinin, a peptide with various physiological effects and antimicrobial properties against bacteria and fungi. In contrast, LMWK is not implicated in blood coagulation. Notably, during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, a reduction or depletion of angiotensin converting enzyme 2 (ACE2) leads to an elevation in des-Arg(9)-bradykinin levels, a bioactive bradykinin metabolite associated with lung injury and inflammation. The gene exhibits restricted expression primarily in the liver (RPKM 432.8).

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC and the IC₅₀ value is < 7 nM.

Human

HEK293

C-His

NP_001095886.1 (Q19-S644)

3827  [NCBI]

Full Length of Mature Protein

QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTVSPPHTSMAPAQDEERDSGKEQGHTRRHDWGHEKQRKHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNGKHNGWKTEHLASSSEDSTTPSAQTQEKTEGPTPIPSLAKPGVTVTFSDFQDSDLIATMMPPISPAPIQSDDDWIPDIQIDPNGLSFNPISDFPDTTSPKCPGRPWKSVSEINPTTQMKESYYFDLTDGLS

100-110 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris, 100 mM NaCl, pH 7.5, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.