LAIR1 Protein, Human (Biotinylated, HEK293, Avi-His)

LAIR1 protein has a negative regulatory effect on NK cells, B cells and T cells. It activates the phosphatases PTPN6 and PTPN11, inhibits calcium increases in B cells, and regulates cytokine production in CD4+ T cells. LAIR1 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived LAIR1 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

The LAIR1 Protein serves as an inhibitory receptor, exerting a constitutive negative regulatory influence on the cytolytic function of natural killer (NK) cells, B-cells, and T-cells. Upon activation through tyrosine phosphorylation, it recruits and activates phosphatases PTPN6 and PTPN11. LAIR1 also dampens the increase in intracellular calcium triggered by B-cell receptor ligation. Beyond its dependency on SH2-containing phosphatases, it independently modulates cytokine production in CD4+ T-cells, suppressing IL2 and IFNG while promoting the secretion of transforming growth factor beta. Additionally, LAIR1 down-regulates IgG and IgE production in B-cells and hinders the secretion of IL8, IL10, and TNF. In myeloid leukemia cell lines, LAIR1 inhibits proliferation, induces apoptosis, and prevents nuclear translocation of NF-kappa-B p65 subunit/RELA, along with the phosphorylation of I-kappa-B alpha/CHUK. Moreover, LAIR1 inhibits the differentiation of peripheral blood precursors into dendritic cells. It interacts with the SH2 domains of tyrosine-protein phosphatases PTPN6 and PTPN11, with the interaction with PTPN6 being constitutive. Furthermore, LAIR1 binds with high affinity to extracellular matrix collagens, highlighting the functional importance of this interaction.

Human

HEK293

C-Avi;C-6*His

AAH27899.1 (Q22-H163)

3903  [NCBI]

Extracellular Domain

Biotin

QEEDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERESRSTYNDTEDVSQASPSESEARFRIDSVSEGNAGPYRCIYYKPPKWSEQSDYLELLVKETSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEH

30-35 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.