SCARB2/LIMP-2 Protein, Mouse (HEK293, His)

LIMPII/SR-B2 Protein acts as a lysosomal receptor crucial for targeting glucosylceramidase (GBA1) to lysosomes. This interaction highlights its role in intracellular trafficking, ensuring the specific recognition and delivery of GBA1 to the lysosomal environment. The protein's direct interaction with GBA1 contributes to essential cellular processes related to lipid metabolism and degradation. SCARB2/LIMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LIMPII/SR-B2 protein, expressed by HEK293 , with C-6*His labeled tag.

LIMPII/SR-B2 Protein functions as a lysosomal receptor that plays a crucial role in the targeting of glucosylceramidase (GBA1) to the lysosomes. This interaction underscores its involvement in the intracellular trafficking and localization of GBA1 within the lysosomal compartment. The protein directly interacts with GBA1, facilitating the specific recognition and delivery of this enzyme to the lysosomal environment, thereby contributing to essential cellular processes associated with lipid metabolism and degradation.

Measured by its binding ability in a functional ELISA. Immobilized Human GBA (HY-P75786) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse LIMPII. The ED₅₀ for this effect is 749.9 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human GBA (HY-P75786) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse LIMPII. The ED₅₀ for this effect is 749.9 ng/mL.

Mouse

HEK293

C-6*His

O35114 (R27-T432)

12492  [NCBI]

Partial

RVFQKAVDQTIEKNMVLQNGTKVFNSWEKPPLPVYIQFYFFNVTNPEEILQGEIPLLEEVGPYTYRELRNKANIQFGENGTTISAVTNKAYVFERNQSVGDPNVDLIRTINIPLLTVVDLAQLTLLRELIEAMLKAYQQKLFVIHTVHELLWGYKDEILSLVHIFKPDVSPNFGLFYERNGTNDGEYVFLTGEDNYLNFSKIVEWNGKTSLDWWTTDTCNMINGTDGDSFHPLISKDEVLYLFPSDLCRSVHITFSSFENVEGLPAFRYKVPAEILANTSENAGFCIPEGNCMDSGVLNISICKNGAPIIMSFPHFYQADEKFVSAIKGMHPNKEEHESFVDINPLTGIILRGAKRFQINTYVRKLDDFVETGDIRTMVFPVMYLNESVLIDKETANQLKSVINTT

Approximately 65-90 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.