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SCARB2/LIMP-2 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

LIMPII/SR-B2 Protein acts as a lysosomal receptor crucial for targeting glucosylceramidase (GBA1) to lysosomes. This interaction highlights its role in intracellular trafficking, ensuring the specific recognition and delivery of GBA1 to the lysosomal environment. The protein's direct interaction with GBA1 contributes to essential cellular processes related to lipid metabolism and degradation. SCARB2/LIMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LIMPII/SR-B2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SCARB2/LIMP-2 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for SCARB2/LIMP-2 Protein, Mouse (HEK293, His)
  • SCARB2/LIMP-2 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

LIMPII/SR-B2 Protein acts as a lysosomal receptor crucial for targeting glucosylceramidase (GBA1) to lysosomes. This interaction highlights its role in intracellular trafficking, ensuring the specific recognition and delivery of GBA1 to the lysosomal environment. The protein's direct interaction with GBA1 contributes to essential cellular processes related to lipid metabolism and degradation. SCARB2/LIMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LIMPII/SR-B2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

LIMPII/SR-B2 Protein functions as a lysosomal receptor that plays a crucial role in the targeting of glucosylceramidase (GBA1) to the lysosomes. This interaction underscores its involvement in the intracellular trafficking and localization of GBA1 within the lysosomal compartment. The protein directly interacts with GBA1, facilitating the specific recognition and delivery of this enzyme to the lysosomal environment, thereby contributing to essential cellular processes associated with lipid metabolism and degradation.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human GBA (HY-P75786) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse LIMPII. The ED50 for this effect is 749.9 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human GBA (HY-P75786) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse LIMPII. The ED50 for this effect is 749.9 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    O35114 (R27-T432)

  • Gene ID
    12492  [NCBI]
  • Molecular Construction
    • N-term
    • LIMPII (R27-T432)
      Accession # O35114
    • 6*His
    • C-term
  • Protein Length

    Lumenal Domain

  • Synonyms

    SCARB2; CD36 Antigen-Like 2; Prev. CD36L2; LGP85; LIMPII; 85 KDa Lysosomal Sialoglycoprotein Scavenger Receptor Class B, Member 2; Lysosome Membrane Protein 2; Lysosomal Integral Membrane Protein II; HLGP85; Alternative Protein SCARB2; SR-BII; CD36 Antige

  • AA Sequence

    RVFQKAVDQTIEKNMVLQNGTKVFNSWEKPPLPVYIQFYFFNVTNPEEILQGEIPLLEEVGPYTYRELRNKANIQFGENGTTISAVTNKAYVFERNQSVGDPNVDLIRTINIPLLTVVDLAQLTLLRELIEAMLKAYQQKLFVIHTVHELLWGYKDEILSLVHIFKPDVSPNFGLFYERNGTNDGEYVFLTGEDNYLNFSKIVEWNGKTSLDWWTTDTCNMINGTDGDSFHPLISKDEVLYLFPSDLCRSVHITFSSFENVEGLPAFRYKVPAEILANTSENAGFCIPEGNCMDSGVLNISICKNGAPIIMSFPHFYQADEKFVSAIKGMHPNKEEHESFVDINPLTGIILRGAKRFQINTYVRKLDDFVETGDIRTMVFPVMYLNESVLIDKETANQLKSVINTT

  • Predicted Molecular Mass

    47.1 kDa

  • Molecular Weight

    Approximately 65-90 kDa, based on SDS-PAGE under reducing conditions,due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human GBA (HY-P75786) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse LIMPII. The ED50 for this effect is 749.9 ng/mL.

  • SCARB2/LIMP-2 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for SCARB2/LIMP-2 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for SCARB2/LIMP-2 Protein, Mouse (HEK293, His)
  • SCARB2/LIMP-2 Protein, Mouse (HEK293, His) Product Image