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Lumican/LUM Protein, Mouse (HEK293, His)

Cat. No.: HY-P70370
COA Handling Instructions

Lumican/LUM protein binds specifically to laminin. Lumican/LUM Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lumican/LUM protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Lumican/LUM Protein, Mouse (HEK293, His) is 320 a.a., with molecular weight of 40-60 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Lumican/LUM protein binds specifically to laminin. Lumican/LUM Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lumican/LUM protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Lumican/LUM Protein, Mouse (HEK293, His) is 320 a.a., with molecular weight of 40-60 kDa.

Background

Lumican/LUM protein exhibits a specific binding affinity for laminin.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of A375 cells. When 5×104 cells per well are added to LUM coated plates (10 μg/mL, 100 μL/well) 52.73% will adhere after 30 minutes at 37°C.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P51885 (Q19-N338)

Gene ID

17022  [NCBI]

Molecular Construction
N-term
Lumican (Q19-N338)
Accession # P51885
6*His
C-term
Synonyms
rMuLumican, His; Keratan sulfate proteoglycan lumican; KSPG lumican; Lumican
AA Sequence

QYYDYDIPLFMYGQISPNCAPECNCPHSYPTAMYCDDLKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGKVFSKLKQLKKLHINYNNLTESVGPLPKSLQDLQLTNNKISKLGSFDGLVNLTFIYLQHNQLKEDAVSASLKGLKSLEYLDLSFNQMSKLPAGLPTSLLTLYLDNNKISNIPDEYFKRFTGLQYLRLSHNELADSGVPGNSFNISSLLELDLSYNKLKSIPTVNENLENYYLEVNELEKFDVKSFCKILGPLSYSKIKHLRLDGNPLTQSSLPPDMYECLRVANEITVN

Molecular Weight

40-60 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Lumican/LUM Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lumican/LUM Protein, Mouse (HEK293, His)
Cat. No.:
HY-P70370
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