MAD2L1 Protein, Human (His-SUMO)

MAD2L1 is a critical spindle assembly checkpoint component that delays anaphase until correct chromosome alignment. During prophase, it forms a heterotetrameric complex with MAD1L1 at unattached kinetochores, recruiting O-MAD2 and aiding transformation. MAD2L1 Protein, Human (His-SUMO) is the recombinant human-derived MAD2L1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

MAD2L1, a crucial component of the spindle-assembly checkpoint, plays a pivotal role in preventing anaphase onset until proper chromosome alignment is achieved at the metaphase plate. During prometaphase, MAD2L1, in its closed conformation, forms a heterotetrameric complex with MAD1L1 at unattached kinetochores, recruiting open conformation molecules of MAD2L1 (O-MAD2) and facilitating the conversion to the closed conformation. Essential for executing the mitotic checkpoint, MAD2L1 monitors kinetochore-spindle attachment, inhibits the anaphase promoting complex by sequestering CDC20, and ensures chromosomes' alignment before anaphase initiation. MAD2L1 can exist as a monomer, homodimer, or heterodimer with MAD1L1, forming a tetrameric core complex. It interacts with various proteins, including MAD2L1BP, ADAM17/TACE, CDC20, BUB1B, TTK, TPR, UBD, NEK2, and HSF1, contributing to its multifaceted roles in cell cycle regulation and mitotic progression. Interactions with isoforms of MAD1L1 lead to cytoplasmic sequestration, adding an additional layer of complexity to MAD2L1's regulatory functions.

Human

E. coli

N-SUMO;N-6*His

Q13257-1 (A2-D205)

4085  [NCBI]

Full Length of Isoform-1 Mature Protein

ALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND

Approximately 39.4 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HC1,1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.