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MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His)

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Based on 1 Customer Validation

MAdCAM1 is a cell adhesion leukocyte receptor expressed primarily by mucosal venules and plays a critical role in coordinating lymphocyte trafficking into mucosal tissues, particularly Peyer's patches and intestinal lamina propria. This protein exhibits the ability to bind integrins α-4/β-7 and L-selectin, thereby regulating the passage and retention of leukocytes in the mucosal environment. MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived MAdCAM1 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His)
  • Experimental Validation Results for MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His)
  • MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

MAdCAM1 is a cell adhesion leukocyte receptor expressed primarily by mucosal venules and plays a critical role in coordinating lymphocyte trafficking into mucosal tissues, particularly Peyer's patches and intestinal lamina propria. This protein exhibits the ability to bind integrins α-4/β-7 and L-selectin, thereby regulating the passage and retention of leukocytes in the mucosal environment. MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived MAdCAM1 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

Background

Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) is a cell adhesion leukocyte receptor expressed on the endothelial cells of mucosal venules, playing a crucial role in directing lymphocyte traffic into mucosal tissues, particularly the Peyer patches and the intestinal lamina propria. MAdCAM1 serves as a binding partner for both integrin alpha-4/beta-7 and L-selectin, thereby regulating the passage and retention of leukocytes in mucosal tissues. The homodimeric structure of MAdCAM1 contributes to its functional role in mediating adhesion between endothelial cells and circulating immune cells. Notably, isoform 2, which lacks the mucin-like domain, may have a specialized function in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Understanding the features and interactions of MAdCAM1 provides insights into the intricate mechanisms governing lymphocyte trafficking in mucosal tissues.

Verified Bioactivity

Immobilized Biotinylated Human MAdCAM1 at 0.5 μg/mL (100 μL/well) can bind Anti- MAdCAM1 antibody. The ED50 for this effect is 3-6 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Biotinylated Human MAdCAM1 at 0.5 μg/mL (100 μL/well) can bind Anti- MAdCAM1 antibody. The ED50 for this effect is 4.081 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-6*His
  • Accession

    Q4PKD0 (Q19-Q333)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • MAdCAM1 (Q19-Q333)
      Accession # Q4PKD0
    • Avi-6*His
    • C-term
  • Protein Length

    Partial

  • Conjugation

    Biotin

  • Conjugate Method

    The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

  • Synonyms

    MADCAM1; HMAdCAM-1; Mucosal Vascular Addressin Cell Adhesion Molecule 1; MAdCAM-1; Mucosal Addressin Cell Adhesion Molecule 1; Mucosal Addressin Cell Adhesion Molecule-1; MACAM1

  • AA Sequence

    QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGHTQGKVIPTGSSKPAGDQ

  • Predicted Molecular Mass

    35.8 kDa

  • Molecular Weight

    Approximately 55-80 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Biotinylated Human MAdCAM1 at 0.5 μg/mL (100 μL/well) can bind Anti- MAdCAM1 antibody. The ED50 for this effect is 4.081 ng/mL.

  • MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His) Product Image
    1/16
  • Experimental Validation Results for MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His)
  • Experimental Validation Results for MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His)
  • MAdCAM1 Protein, Human (Biotinylated, HEK293, Avi-His) Product Image