MAdCAM1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
MAdCAM1 Protein is an important cell adhesion molecule and a member of the immunoglobulin superfamily. MAdCAM1 Protein plays a key role in immune system cell migration and mucosal immune responses. MAdCAM1 Protein, Human (HEK293, His) is a recombinant MAdCAM1 protein expressed by HEK293, with a C-6*His tag and glycosylation modification.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
MAdCAM1 Protein is an important cell adhesion molecule and a member of the immunoglobulin superfamily. MAdCAM1 Protein plays a key role in immune system cell migration and mucosal immune responses. MAdCAM1 Protein, Human (HEK293, His) is a recombinant MAdCAM1 protein expressed by HEK293, with a C-6*His tag and glycosylation modification[1].
Background
MAdCAM1 is a transmembrane glycoprotein expressed by endothelial cells, primarily located in the vascular endothelium of gut-associated lymphoid tissues and blood vessels at chronic inflammatory sites. MAdCAM1 mediates the homing of lymphocytes to intestinal mucosal tissues and organs such as the liver under inflammatory conditions by binding to the integrin α4β7 on the surface of lymphocytes. MAdCAM1 is upregulated during intestinal inflammation or infection to promote lymphocyte infiltration and enhance local immune defense, and its abnormal expression is closely associated with inflammatory bowel disease[1].
In Vitro
PF-00547659 binds to MAdCAM1 Protein, Human and inhibits the adhesion of α4β7-expressing cells to human and cynomolgus MAdCAM in vitro[2].
Verified Bioactivity
1.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED50 for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.
2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. When 5×104 cells/well are added to Human MAdCAM-1 coated plates (2.5 μg/mL with 100 µL/well), approximately 52.575%-62.728% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl2.
3.Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5x104 cells/well are added to human MAdCAM-1 coated plates (2.5 µg/mL with 100 µL/well), approximately 67.699% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl2.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q4PKD0 (Q19-Q333)
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Gene ID/
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Molecular Construction
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N-term
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MAdCAM1 (Q19-Q333)
Accession # Q4PKD0 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
MADCAM1; HMAdCAM-1; Mucosal Vascular Addressin Cell Adhesion Molecule 1; MAdCAM-1; Mucosal Addressin Cell Adhesion Molecule 1; Mucosal Addressin Cell Adhesion Molecule-1; MACAM1
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AA Sequence
QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGHTQGKVIPTGSSKPAGDQ
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Molecular Weight
Approximately 55-65 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Schippers A, et al. MAdCAM-1/α4β7 Integrin-Mediated Lymphocyte/Endothelium Interactions Exacerbate Acute Immune-Mediated Hepatitis in Mice. Cell Mol Gastroenterol Hepatol. 2021;11(4):1227-1250.e1. [Content Brief]
[2]. Reinisch W, et al. Targeting Endothelial Ligands: ICAM-1/alicaforsen, MAdCAM-1. J Crohns Colitis. 2018 Aug 22;12(suppl_2):S669-S677. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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