1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. MAdCAM-1
  6. MAdCAM1 Protein, Human (HEK293, His)

MAdCAM1 Protein, Human (HEK293, His)

Cat. No.: HY-P70852
COA Handling Instructions

MAdCAM1 is a cell adhesion leukocyte receptor expressed primarily by mucosal venules and plays a critical role in coordinating lymphocyte trafficking into mucosal tissues, particularly Peyer's patches and intestinal lamina propria. This protein exhibits the ability to bind integrins α-4/β-7 and L-selectin, thereby regulating the passage and retention of leukocytes in the mucosal environment. MAdCAM1 Protein, Human (HEK293, His) is the recombinant human-derived MAdCAM1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAdCAM1 Protein, Human (HEK293, His) is 315 a.a., with molecular weight of 55-65 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

MAdCAM1 is a cell adhesion leukocyte receptor expressed primarily by mucosal venules and plays a critical role in coordinating lymphocyte trafficking into mucosal tissues, particularly Peyer's patches and intestinal lamina propria. This protein exhibits the ability to bind integrins α-4/β-7 and L-selectin, thereby regulating the passage and retention of leukocytes in the mucosal environment. MAdCAM1 Protein, Human (HEK293, His) is the recombinant human-derived MAdCAM1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAdCAM1 Protein, Human (HEK293, His) is 315 a.a., with molecular weight of 55-65 kDa.

Background

Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) is a cell adhesion leukocyte receptor expressed on the endothelial cells of mucosal venules, playing a crucial role in directing lymphocyte traffic into mucosal tissues, particularly the Peyer patches and the intestinal lamina propria. MAdCAM1 serves as a binding partner for both integrin alpha-4/beta-7 and L-selectin, thereby regulating the passage and retention of leukocytes in mucosal tissues. The homodimeric structure of MAdCAM1 contributes to its functional role in mediating adhesion between endothelial cells and circulating immune cells. Notably, isoform 2, which lacks the mucin-like domain, may have a specialized function in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Understanding the features and interactions of MAdCAM1 provides insights into the intricate mechanisms governing lymphocyte trafficking in mucosal tissues.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED50 for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED50 for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q4PKD0 (Q19-Q333)

Gene ID

/

Molecular Construction
N-term
MAdCAM1 (Q19-Q333)
Accession # Q4PKD0
6*His
C-term
Synonyms
hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1
AA Sequence

QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGHTQGKVIPTGSSKPAGDQ

Molecular Weight

55-65 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

MAdCAM1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAdCAM1 Protein, Human (HEK293, His)
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HY-P70852
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