1. Recombinant Proteins
  2. Receptor Proteins
  3. Siglec
  4. Myelin Associated Glycoprotein (MAG/Siglec-4a)
  5. MAG/Siglec-4a Protein, Human (HEK293, His)

MAG/Siglec-4a Protein, Human (HEK293, His)

Cat. No.: HY-P70318
COA Handling Instructions

MAG/Siglec-4a is an adhesion molecule that binds to neuronal gangliosides, RTN4R, and RTN4RL2, promoting myelination interactions. Although not important for initial myelination, it may maintain axonal myelination and protect motor neurons, especially after injury, through interactions with RTN4R and RTN4RL2. MAG/Siglec-4a Protein, Human (HEK293, His) is the recombinant human-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

MAG/Siglec-4a is an adhesion molecule that binds to neuronal gangliosides, RTN4R, and RTN4RL2, promoting myelination interactions. Although not important for initial myelination, it may maintain axonal myelination and protect motor neurons, especially after injury, through interactions with RTN4R and RTN4RL2. MAG/Siglec-4a Protein, Human (HEK293, His) is the recombinant human-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The MAG/Siglec-4a protein serves as an adhesion molecule facilitating interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides, as well as the glycoproteins RTN4R and RTN4RL2. Although not essential for initial myelination, MAG/Siglec-4a appears to play a crucial role in maintaining normal axon myelination and protecting motoneurons against apoptosis, particularly after injury. This protective effect is likely mediated through interactions with neuronal RTN4R and RTN4RL2. In adults, MAG/Siglec-4a is required to prevent degeneration of myelinated axons, possibly relying on binding to gangliosides on the axon cell membrane. Acting as a negative regulator of neurite outgrowth, MAG/Siglec-4a inhibits axon longitudinal growth and outgrowth by preferentially binding to alpha-2,3-linked sialic acid and interacting with RTN4R, RTN4RL2, and gangliosides. The protein exists as both a monomer and homodimer, and its interactions extend to include isoform 2 of BSG, contributing to its intricate role in modulating neuronal responses and axonal dynamics.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P20916-1 (G20-P516)

Gene ID
Molecular Construction
N-term
MAG (G20-P516)
Accession # P20916-1
6*His
C-term
Synonyms
rHuMyelin-associated glycoprotein/MAG, His; Myelin-Associated Glycoprotein; Siglec-4a; MAG; GMA
AA Sequence

GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

Molecular Weight

84-108 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

MAG/Siglec-4a Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAG/Siglec-4a Protein, Human (HEK293, His)
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HY-P70318
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