MAG/Siglec-4a Protein, Human (HEK293, His)
Based on 1 Customer Validation
MAG/Siglec-4a is an adhesion molecule that binds to neuronal gangliosides, RTN4R, and RTN4RL2, promoting myelination interactions. Although not important for initial myelination, it may maintain axonal myelination and protect motor neurons, especially after injury, through interactions with RTN4R and RTN4RL2. MAG/Siglec-4a Protein, Human (HEK293, His) is the recombinant human-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MAG/Siglec-4a is an adhesion molecule that binds to neuronal gangliosides, RTN4R, and RTN4RL2, promoting myelination interactions. Although not important for initial myelination, it may maintain axonal myelination and protect motor neurons, especially after injury, through interactions with RTN4R and RTN4RL2. MAG/Siglec-4a Protein, Human (HEK293, His) is the recombinant human-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The MAG/Siglec-4a protein serves as an adhesion molecule facilitating interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides, as well as the glycoproteins RTN4R and RTN4RL2. Although not essential for initial myelination, MAG/Siglec-4a appears to play a crucial role in maintaining normal axon myelination and protecting motoneurons against apoptosis, particularly after injury. This protective effect is likely mediated through interactions with neuronal RTN4R and RTN4RL2. In adults, MAG/Siglec-4a is required to prevent degeneration of myelinated axons, possibly relying on binding to gangliosides on the axon cell membrane. Acting as a negative regulator of neurite outgrowth, MAG/Siglec-4a inhibits axon longitudinal growth and outgrowth by preferentially binding to alpha-2,3-linked sialic acid and interacting with RTN4R, RTN4RL2, and gangliosides. The protein exists as both a monomer and homodimer, and its interactions extend to include isoform 2 of BSG, contributing to its intricate role in modulating neuronal responses and axonal dynamics.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P20916-1 (G20-P516)
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Molecular Construction
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N-term
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MAG (G20-P516)
Accession # P20916-1 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
MAG; S-MAG; Prev. GMA; Myelin-Associated Glycoprotein; SIGLEC-4A; Sialic Acid-Binding Immunoglobulin-Like Lectin 4A; SIGLEC4A; Siglec-4a; SIGLEC4; SPG75; Myelin Associated Glycoprotein; Sialic Acid Binding Ig-Like Lectin 4A
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AA Sequence
GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP
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Predicted Molecular Mass
55.7 kDa
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Molecular Weight
Approximately 65-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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