MAP2K7 Protein, Human (sf9, His, GST)
Based on 1 Customer Validation
MAP2K7 protein is a dual-specificity kinase involved in the MAP kinase signal transduction pathway. MAP2K7 Protein, Human (sf9, His, GST) is the recombinant human-derived MAP2K7 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MAP2K7 protein is a dual-specificity kinase involved in the MAP kinase signal transduction pathway. MAP2K7 Protein, Human (sf9, His, GST) is the recombinant human-derived MAP2K7 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Background
MAP2K7, a dual specificity protein kinase, plays a pivotal role as an essential component in the MAP kinase signal transduction pathway, particularly in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling cascade. Teaming up with MAP2K4/MKK4, MAP2K7/MKK7 directly activates JNK family members, including MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3, by phosphorylating the critical Thr-Pro-Tyr motif. Notably, MAP2K7/MKK7 exhibits a preference for phosphorylating the Thr residue, while MAP2K4/MKK4 favors the Tyr residue. This distinction ensures optimal JNK activation, as the monophosphorylation of JNKs on the Thr residue alone is sufficient to enhance their activity. MAP2K7/MKK7's specific involvement in the JNK signaling pathway activated by pro-inflammatory cytokines contributes to cellular responses, including mitochondrial death signaling leading to apoptosis. Furthermore, MAP2K7 participates in a non-canonical MAPK signaling pathway alongside MAP3K12, MAPK1/ERK2, and MAPK3/ERK1, enhancing AP-1-mediated transcription of APP in response to APOE.
Verified Bioactivity
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His;N-GST
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Accession
O14733 (A2-R419)
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Gene ID5609
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Molecular Construction
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N-term
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8*His-GST
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MAP2K7 (A2-R419)
Accession # O14733 -
C-term
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Protein Length
Full length
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Synonyms
MAP2K7; SAPK Kinase 4; Prev. PRKMK7; MEK 7; SAPKK4; JNK Kinase 2; MKK7; MAPKK 7; Dual Specificity Mitogen-Activated Protein Kinase Kinase 7; MAPKK7; Stress-Activated Protein Kinase Kinase 4; JNKK 2; Jnkk2; JNKK2; C-Jun N-Terminal Kinase Kinase 2; SKK4; JN
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AA Sequence
AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQHPTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQAEINDLENLGEMGSGTCGQVWKMRFRKTGHVIAVKQMRRSGNKEENKRILMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGPIPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPHLPFFR
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Predicted Molecular Mass
75 kDa
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Molecular Weight
Approximately 75 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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