MAP2K7 Protein, Human (sf9, His, GST)

MAP2K7 protein is a dual-specificity kinase involved in the MAP kinase signal transduction pathway. MAP2K7 Protein, Human (sf9, His, GST) is the recombinant human-derived MAP2K7 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

MAP2K7, a dual specificity protein kinase, plays a pivotal role as an essential component in the MAP kinase signal transduction pathway, particularly in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling cascade. Teaming up with MAP2K4/MKK4, MAP2K7/MKK7 directly activates JNK family members, including MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3, by phosphorylating the critical Thr-Pro-Tyr motif. Notably, MAP2K7/MKK7 exhibits a preference for phosphorylating the Thr residue, while MAP2K4/MKK4 favors the Tyr residue. This distinction ensures optimal JNK activation, as the monophosphorylation of JNKs on the Thr residue alone is sufficient to enhance their activity. MAP2K7/MKK7's specific involvement in the JNK signaling pathway activated by pro-inflammatory cytokines contributes to cellular responses, including mitochondrial death signaling leading to apoptosis. Furthermore, MAP2K7 participates in a non-canonical MAPK signaling pathway alongside MAP3K12, MAPK1/ERK2, and MAPK3/ERK1, enhancing AP-1-mediated transcription of APP in response to APOE.

Human

Sf9 insect cells

N-8*His;N-GST

O14733 (A2-R419)

5609

Full length

AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQHPTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQAEINDLENLGEMGSGTCGQVWKMRFRKTGHVIAVKQMRRSGNKEENKRILMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGPIPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPHLPFFR

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.