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Melanotransferrin/CD228 Protein, Human (HEK293, His)

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Melanotransferrin, or CD228, is vital for cellular iron uptake, binding a single iron atom per subunit. The protein is involved in internalization and recycling to the cell membrane, with additional potential versatility in zinc binding. Melanotransferrin/CD228 Protein, Human (HEK293, His) is the recombinant human-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Melanotransferrin/CD228 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Melanotransferrin, or CD228, is vital for cellular iron uptake, binding a single iron atom per subunit. The protein is involved in internalization and recycling to the cell membrane, with additional potential versatility in zinc binding. Melanotransferrin/CD228 Protein, Human (HEK293, His) is the recombinant human-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag.

Background

Melanotransferrin, also known as CD228, plays a crucial role in cellular iron uptake by binding a single atom of iron per subunit. The protein is implicated in the internalization and subsequent recycling back to the cell membrane. Notably, melanotransferrin exhibits the capacity to bind zinc, suggesting potential versatility in metal ion interactions.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    P08582-1 (G20-C709)

  • Gene ID
    4241  [NCBI]
  • Molecular Construction
    • N-term
    • Melanotransferrin (G20-C709)
      Accession # P08582-1
    • 8*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    MELTF; Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 And 96.5; Prev. MFI2; FLJ38863; MAP97; MGC4856; CD228; Melanoma-Associated Antigen P97; MTF1; CD228 Antigen; MTf; Melanotransferrin; Membrane-Bound Transferrin-Like Protein

  • AA Sequence

    GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

  • Predicted Molecular Mass

    76.6 kDa

  • Molecular Weight

    Approximately 78-88 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Melanotransferrin/CD228 Protein, Human (HEK293, His) Product Image