2. Recombinant Proteins
  3. CD Antigens
  4. Epithelial cell CD Proteins
  5. CD228
  6. Melanotransferrin/CD228 Protein, Human (HEK293, His)

Melanotransferrin/CD228 Protein, Human (HEK293, His)

Cat. No.: HY-P700671
Handling Instructions

Melanotransferrin, or CD228, is vital for cellular iron uptake, binding a single iron atom per subunit. The protein is involved in internalization and recycling to the cell membrane, with additional potential versatility in zinc binding. Melanotransferrin/CD228 Protein, Human (HEK293, His) is the recombinant human-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag. The total length of Melanotransferrin/CD228 Protein, Human (HEK293, His) is 690 a.a., with molecular weight of 78-88 kDa.

For research use only. We do not sell to patients.

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Description

Melanotransferrin, or CD228, is vital for cellular iron uptake, binding a single iron atom per subunit. The protein is involved in internalization and recycling to the cell membrane, with additional potential versatility in zinc binding. Melanotransferrin/CD228 Protein, Human (HEK293, His) is the recombinant human-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag. The total length of Melanotransferrin/CD228 Protein, Human (HEK293, His) is 690 a.a., with molecular weight of 78-88 kDa.
Background

Melanotransferrin, also known as CD228, plays a crucial role in cellular iron uptake by binding a single atom of iron per subunit. The protein is implicated in the internalization and subsequent recycling back to the cell membrane. Notably, melanotransferrin exhibits the capacity to bind zinc, suggesting potential versatility in metal ion interactions.
Species

Human
Source

HEK293
Tag

C-His
Accession

P08582-1 (G20-C709)
Gene ID

/
Molecular Construction
N-term
Melanotransferrin (G20-C709)
Accession # P08582-1
His
C-term
Synonyms
Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
Molecular Weight

78-88 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Melanotransferrin/CD228 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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