METTL14-METTL3 Protein, Human (sf9, His, Strep)
Based on 1 Customer Validation
METTL3 and METTL14 are important players in RNA methylation specifically involved in N6-methyladenosine (m6A) modification. METTL3 and METTL14 have shown associations with certain cancers and neurological disorders. METTL14-METTL3 Protein, Human (sf9, His, Strep) is expressed by Sf9 insect cells and carries Strep and His tags.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
METTL3 and METTL14 are important players in RNA methylation specifically involved in N6-methyladenosine (m6A) modification. METTL3 and METTL14 have shown associations with certain cancers and neurological disorders. METTL14-METTL3 Protein, Human (sf9, His, Strep) is expressed by Sf9 insect cells and carries Strep and His tags[1].
Background
N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. In mammalian cells, METTL3 (methyltransferase-like 3) and METTL14 (methyltransferase-like 14) are two m6A methyltransferases reported to date, which both contain methyltransferase domains. These two proteins form a stable heterodimer core complex of METTL3–METTL14 that functions in cellular m6A deposition on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. They modulate various RNA biological processes impacting cellular response and development. In various cancers such as acute myeloid leukemia upregulated METTL3 levels correlate with increased malignancy and poor prognosis. In neurological contexts altered m6A modification patterns from METTL3 and METTL14 activity may connect to proteins like FTO which has implications in neurodevelopmental disorders[1].
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Strep;His
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Accession
Q9HCE5 (D2-R456)&Q86U44-1 (S2-L580)
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Gene ID57721
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Synonyms
METTL14; Methyltransferase Like 14; Methyltransferase 14, N6-Adenosine-Methyltransferase Non-Catalytic Subunit; HMETTL14; N(6)-Adenosine-Methyltransferase Non-Catalytic Subunit METTL14; Methyltransferase 14, N6-Adenosine-Methyltransferase Complex Catalyti
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Predicted Molecular Mass
53.9 kDa & 66.1 kDa
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Molecular Weight
Approximately 60 kDa & 70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (260 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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