METTL14-METTL3 Protein, Human (sf9, His, Strep)

METTL3 and METTL14 are important players in RNA methylation specifically involved in N6-methyladenosine (m6A) modification. METTL3 and METTL14 have shown associations with certain cancers and neurological disorders. METTL14-METTL3 Protein, Human (sf9, His, Strep) is expressed by Sf9 insect cells and carries Strep and His tags^[1].

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. In mammalian cells, METTL3 (methyltransferase-like 3) and METTL14 (methyltransferase-like 14) are two m6A methyltransferases reported to date, which both contain methyltransferase domains. These two proteins form a stable heterodimer core complex of METTL3–METTL14 that functions in cellular m6A deposition on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. They modulate various RNA biological processes impacting cellular response and development. In various cancers such as acute myeloid leukemia upregulated METTL3 levels correlate with increased malignancy and poor prognosis. In neurological contexts altered m6A modification patterns from METTL3 and METTL14 activity may connect to proteins like FTO which has implications in neurodevelopmental disorders^[1].

Human

Sf9 insect cells

Strep;His

Q9HCE5 (D2-R456)&Q86U44-1 (S2-L580)

57721

Approximately 60 kDa & 70 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Zeng C, et al. Roles of METTL3 in cancer: mechanisms and therapeutic targeting. J Hematol Oncol. 2020 Aug 27;13(1):117.  [Content Brief]