MRCKα Protein, Human (Active, sf9, GST)
The MRCKα protein is a key serine/threonine protein kinase downstream of CDC42 that coordinates cytoskeletal dynamics and cell migration. MRCKα phosphorylates PPP1R12C and MYL9/MLC2, regulating actin reorganization. MRCKα Protein, Human (sf9, GST) is the recombinant human-derived MRCKα protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The MRCKα protein is a key serine/threonine protein kinase downstream of CDC42 that coordinates cytoskeletal dynamics and cell migration. MRCKα phosphorylates PPP1R12C and MYL9/MLC2, regulating actin reorganization. MRCKα Protein, Human (sf9, GST) is the recombinant human-derived MRCKα protein, expressed by sf9 insect cells , with N-GST labeled tag.
Background
MRCKα Protein, a serine/threonine-protein kinase, serves as a crucial downstream effector of CDC42, playing a pivotal role in orchestrating cytoskeleton reorganization and cell migration. Through phosphorylation of PPP1R12C and MYL9/MLC2, MRCKα actively regulates actin cytoskeletal reorganization. Teaming up with MYO18A and LURAP1, it contributes to the modulation of lamellar actomyosin retrograde flow, a process essential for cell protrusion and migration. MRCKα also phosphorylates PPP1R12A, LIMK1, and LIMK2, influencing various aspects of cellular dynamics. Furthermore, it may be involved in TFRC-mediated iron uptake and, in collaboration with FAM89B/LRAP25, facilitates the targeting of LIMK1 to the lamellipodium, leading to its activation and subsequent phosphorylation of CFL1—an event crucial for the regulation of lamellipodial F-actin. Additionally, MRCKα triggers the formation of an extrusion apical actin ring, playing a vital role in the epithelial extrusion of apoptotic cells.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q5VT25 (S2-Q473)
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Gene ID8476
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Molecular Construction
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N-term
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GST
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MRCKα (S2-Q473)
Accession # Q5VT25 -
C-term
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Protein Length
Partial
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Synonyms
CDC42BPA; Myotonic Dystrophy Kinase-Related CDC42-Binding Protein Kinase Alpha; CDC42 Binding Protein Kinase Alpha; Myotonic Dystrophy Kinase-Related CDC42-Binding Kinase Alpha; Serine/Threonine-Protein Kinase MRCK Alpha; Myotonic Dystrophy Kinase-Related
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AA Sequence
SGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQ
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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