MYLK Protein, Human (Inactive, sf9, His, GST)

MYLK Protein, Human (Inactive, sf9, His, GST) is the recombinant human-derived MYLK, expressed by sf9 insect cells , with His, GST labeled tag. ,

MYLK is a calcium/calmodulin-dependent myosin light chain kinase that regulates smooth muscle contraction by phosphorylating myosin light chains (MLC). It also modulates actin-myosin interaction through a non-kinase activity and phosphorylates PTK2B/PYK2 and myosin light chains. This protein plays roles in inflammatory responses (e.g., apoptosis, vascular permeability, leukocyte diapedesis), cell motility/morphology, airway hyperreactivity, and asthma-related processes. It is essential for tonic airway smooth muscle contraction underlying physiological and asthmatic airway resistance, as well as gastrointestinal motility. MYLK likely regulates endothelial/vascular permeability via cytoskeletal rearrangements. In the nervous system, it controls astrocytic process growth in culture and participates in synaptic transmitter release. As a critical mediator of fibroblast apoptosis signaling, MYLK also influences epithelial cell survival, particularly during wound healing by mediating actomyosin ring contraction. It induces RhoA-dependent membrane blebbing and activates TRPC5 channels via calcium-dependent plasma membrane localization, promoting cell (including tumor) migration and metastasis. Phosphorylation of PTK2B/PYK2 by MYLK activates ITGB2, enabling neutrophil transmigration in acute lung injury (ALI). MYLK may regulate optic nerve head astrocyte migration and mitotic cytoskeletal organization, while modulating tight junctions through ZO-1 exchange. In burn injuries, it mediates microvascular barrier dysfunction by phosphorylating MLC to induce endothelial contraction. It is crucial for intestinal barrier impairment and Giardia spp.-induced epithelial dysfunction via F-actin/ZO-1 reorganization. MYLK enables hypotonicity-induced Ca²⁺ entry and volume-sensitive organic osmolyte/anion channel (VSOAC) activation in cervical cancer cells and supports breast cancer cell proliferation via anti-apoptotic mechanisms.

The product demonstrated no detectable kinase activity measured by Caliper MSA kinase assay.

Human

Sf9 insect cells

His;GST

Q15746-1 (G1425-S1776)

4638

Partial

GEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGS

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.