NCAM-1/CD56 Protein, Human (HEK293, His-Avi)

Neural cell adhesion molecule 1 (NCAM1) is a cell adhesion molecule which enables LRR domain binding activity and phosphatase binding activity. NCAM1 plays a role in the cell development and differentiation as well as immune surveillance. NCAM1 plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and activating FAK, MAPK and PI3K. NCAM-1/CD56 Protein, Human (HEK293, His-Avi) is the recombinant human-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Neural cell adhesion molecule 1 (NCAM1) is a cell adhesion molecule which is a member of the immunoglobulin superfamily and enables LRR domain binding activity and phosphatase binding activity, participating homotypic cell-cell adhesion, positive regulation of calcium-mediated signaling, and regulation of exocyst assembly.
NCAM1 is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation, playing a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. NCAM1 is also involved in commissural neuron axon guidance and regulation of semaphorin-plexin signaling pathway. Moreover, NCAM1 is associated with the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. NCAM1 plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K).
NCAM1 gene has multiple protein isoforms through alternative splicing. One prominent isoform of NCAM1 is cell surface molecule CD56, which plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of CD56 is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood.
NCAM1 also acts as a receptor for rabies virus and Zika virus^[1][2].

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.7567 μg/mL, corresponding to a specific activity is 1.32×10^3 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.7567 μg/mL, corresponding to a specific activity is 1.32×10³ units/mg.

Human

HEK293

C-Avi;C-His

P13591-3 (L20-P603)

4684  [NCBI]

Partial

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVHSPPP

Approximately 100 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Overexpression of murine Pax3 increases NCAM polysialylation in a human medulloblastoma cell line. J Biol Chem. 2000 Jul 28;275(30):23259-66.  [Content Brief]

  [2]. Angata K, et al. Differential biosynthesis of polysialic acid on neural cell adhesion molecule (NCAM) and oligosaccharide acceptors by three distinct alpha 2,8-sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III. J Biol Chem. 2000 Jun 16;275(24):18594-601.  [Content Brief]