NCAM-1/CD56 Protein, Rat (HEK293, His)

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

NCAM-1/CD56 Protein, a cell adhesion molecule, plays a pivotal role in various neuronal processes, including neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurites. It interacts with MDK, contributing to the intricate cellular interactions in neural development. Furthermore, NCAM-1 is part of a complex involving SLC39A6, SLC39A10, and NCAM1 itself, which regulates NCAM1 phosphorylation and facilitates its integration into focal adhesion complexes during epithelial-to-mesenchymal transition. This underscores the dynamic involvement of NCAM-1 in cell adhesion and signaling events, emphasizing its importance in both neural development and cellular processes associated with epithelial-to-mesenchymal transition.

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×10³ units/mg.

Rat

HEK293

C-His

P13596 (L20-T721)

24586  [NCBI]

Extracellular Domain

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

115-130 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris and 0.15 M NaCl, pH 8.0, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.