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NCAM-1/CD56 Protein, Rat (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NCAM-1/CD56 Protein, Rat (HEK293, His)
  • Experimental Validation Results for NCAM-1/CD56 Protein, Rat (HEK293, His)
  • NCAM-1/CD56 Protein, Rat (HEK293, His) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

Background

NCAM-1/CD56 Protein, a cell adhesion molecule, plays a pivotal role in various neuronal processes, including neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurites. It interacts with MDK, contributing to the intricate cellular interactions in neural development. Furthermore, NCAM-1 is part of a complex involving SLC39A6, SLC39A10, and NCAM1 itself, which regulates NCAM1 phosphorylation and facilitates its integration into focal adhesion complexes during epithelial-to-mesenchymal transition. This underscores the dynamic involvement of NCAM-1 in cell adhesion and signaling events, emphasizing its importance in both neural development and cellular processes associated with epithelial-to-mesenchymal transition.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED50 this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED50 this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×103 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    P13596 (L20-T721)

  • Gene ID
    24586  [NCBI]
  • Molecular Construction
    • N-term
    • NCAM-1 (L20-T721)
      Accession # P13596
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NCAM1; Alternative Protein NCAM1; Neural Cell Adhesion Molecule 1; CD56 Antigen; NCAM; N-CAM-1; CD56; NCAM-1; Antigen Recognized By Monoclonal Antibody 5.1H11; MSK39; Neural Cell Adhesion Molecule, NCAM

  • AA Sequence

    LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

  • Molecular Weight

    Approximately 114-118 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris and 0.15 M NaCl, pH 8.0, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED50 this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×103 units/mg.

  • NCAM-1/CD56 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for NCAM-1/CD56 Protein, Rat (HEK293, His)
  • Experimental Validation Results for NCAM-1/CD56 Protein, Rat (HEK293, His)
  • NCAM-1/CD56 Protein, Rat (HEK293, His) Product Image