1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Neuregulins
  5. Neuregulin-4 (NRG4)
  6. Neuregulin-4/NRG4 Protein, Human

Neuregulin-4/NRG4 Protein, Human

Cat. No.: HY-P76524
COA Handling Instructions

Neuregulin-4 (NRG4) Protein, a low-affinity ligand for the ERBB4 tyrosine kinase receptor, serves as a signaling molecule that recruits ERBB1 and ERBB2 coreceptors. This recruitment leads to ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. NRG4 specifically interacts with the ERBB4 receptor, mediating cellular responses through its activation, without binding to ERBB1, ERBB2, and ERBB3 receptors. Neuregulin-4/NRG4 Protein, Human is the recombinant human-derived Neuregulin-4/NRG4 protein, expressed by E. coli , with tag free. The total length of Neuregulin-4/NRG4 Protein, Human is 61 a.a., with molecular weight of ~6.7 KDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Neuregulin-4 (NRG4) Protein, a low-affinity ligand for the ERBB4 tyrosine kinase receptor, serves as a signaling molecule that recruits ERBB1 and ERBB2 coreceptors. This recruitment leads to ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. NRG4 specifically interacts with the ERBB4 receptor, mediating cellular responses through its activation, without binding to ERBB1, ERBB2, and ERBB3 receptors. Neuregulin-4/NRG4 Protein, Human is the recombinant human-derived Neuregulin-4/NRG4 protein, expressed by E. coli , with tag free. The total length of Neuregulin-4/NRG4 Protein, Human is 61 a.a., with molecular weight of ~6.7 KDa.

Background

Neuregulin-4 (NRG4), a low-affinity ligand for the ERBB4 tyrosine kinase receptor, acts as a signaling molecule that simultaneously recruits ERBB1 and ERBB2 coreceptors. This recruitment leads to ligand-stimulated tyrosine phosphorylation and subsequent activation of the ERBB receptors. Notably, NRG4 does not bind to the ERBB1, ERBB2, and ERBB3 receptors. It specifically interacts with the ERBB4 receptor, playing a role in mediating cellular responses through the activation of this particular receptor.

Biological Activity

Measured in a cell proliferation assay using Raji human Burkitt's lymphoma cells. The ED50 for this effect is 0.0144 μg/mL, corresponding to a specific activity is 6.944×104 units/mg.

  • Measured in a cell proliferation assay using Raji human Burkitt's lymphoma cells. The ED50 this effect is 0.0144 μg/mL, corresponding to a specific activity is 6.944×10^4 units/mg.
Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q8WWG1 (GP&P2-F62)

Gene ID
Synonyms
Pro-neuregulin-4, membrane-bound isoform; Pro-NRG4; NRG-4
AA Sequence

GPPTDHEEPCGPSHKSFCLNGGLCYVIPTIPSPFCRCVENYTGARCEEVFLPGSSIQTKSNLF

Molecular Weight

Approximately 6.7 kDa.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, pH 7.2 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Neuregulin-4/NRG4 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Neuregulin-4/NRG4 Protein, Human
Cat. No.:
HY-P76524
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