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  3. Nidogen-1 Protein, Mouse (P.pastoris, His)

Nidogen-1 Protein, Mouse (P.pastoris, His)

Cat. No.: HY-P71763
COA Handling Instructions

Nidogen-1 protein is a sulfated glycoprotein that is widely distributed in basement membranes and binds tightly to laminin, type IV collagen, and perlecan, suggesting multiple interactions between cells and the extracellular matrix .Nidogen-1 binds to partners such as FBLN1, LGALS3BP and PLXDC1 and plays a crucial role in the complex molecular network of the extracellular matrix.Nidogen-1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Nidogen-1 protein, expressed by P.pastoris , with N-His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $225 In-stock
50 μg $425 In-stock
100 μg $680 In-stock
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  • Biological Activity

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Description

Nidogen-1 protein is a sulfated glycoprotein that is widely distributed in basement membranes and binds tightly to laminin, type IV collagen, and perlecan, suggesting multiple interactions between cells and the extracellular matrix .Nidogen-1 binds to partners such as FBLN1, LGALS3BP and PLXDC1 and plays a crucial role in the complex molecular network of the extracellular matrix.Nidogen-1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Nidogen-1 protein, expressed by P.pastoris , with N-His labeled tag.

Background

Nidogen-1 protein, a sulfated glycoprotein, exhibits a broad distribution within basement membranes and forms a close association with laminin. This versatile protein extends its binding capabilities to collagen IV and perlecan, implying its involvement in diverse cell-extracellular matrix interactions. Nidogen-1 plays a pivotal role in mediating these interactions by engaging with various partners, including FBLN1, LGALS3BP, and PLXDC1. Its associations with these proteins highlight its multifaceted involvement in the complex network of molecular interactions within the extracellular matrix. These interactions likely contribute to the regulation of cellular processes and structural integrity in various tissues and underscore the importance of nidogen-1 in maintaining the dynamic equilibrium of the extracellular microenvironment.

Species

Mouse

Source

P. pastoris

Tag

N-His

Accession

P10493 (428S-665A)

Gene ID
Molecular Construction
N-term
His
Nidogen-1 (428S-665A)
Accession # P10493
C-term
Synonyms
Nid1; Ent; Nidogen-1; NID-1; Entactin
AA Sequence

SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA

Molecular Weight

Approximately 28.3 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Nidogen-1 Protein, Mouse (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nidogen-1 Protein, Mouse (P.pastoris, His)
Cat. No.:
HY-P71763
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