1. Recombinant Proteins
  2. CD Antigens Receptor Proteins Biotinylated Proteins
  3. Platelet CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. P-Selectin/CD62P Selectin
  5. P-Selectin/CD62P
  6. P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His)

P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His)

Cat. No.: HY-P72408
SDS COA Handling Instructions

P-selectin is a Ca(2+)-dependent receptor on myeloid cells that specifically binds to carbohydrates on neutrophils and monocytes.It mediates interactions between activated endothelial cells or platelets and leukocytes by recognizing sialic acid-Lewis X.P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His) is the recombinant mouse-derived P-selectin protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $300 In-stock
100 μg $750 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

P-selectin is a Ca(2+)-dependent receptor on myeloid cells that specifically binds to carbohydrates on neutrophils and monocytes.It mediates interactions between activated endothelial cells or platelets and leukocytes by recognizing sialic acid-Lewis X.P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His) is the recombinant mouse-derived P-selectin protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

Background

The P-selectin protein is a Ca(2+)-dependent receptor found on myeloid cells, which specifically binds to carbohydrates on neutrophils and monocytes. It plays a crucial role in mediating the interaction between activated endothelial cells or platelets and leukocytes. The ligand recognized by P-selectin is sialyl-Lewis X. P-selectin facilitates the rapid rolling of leukocytes over vascular surfaces during the initial stages of inflammation by interacting with SELPLG. It also interacts with SNX17. Moreover, P-selectin interacts with SELPLG/PSGL1 and PODXL2, promoting neutrophil adhesion and leukocyte rolling. This interaction requires the presence of the sialyl-Lewis X epitope on SELPLG and PODXL2, as well as specific tyrosine sulfation on SELPLG.

Species

Mouse

Source

HEK293

Tag

C-Avi;C-6*His

Accession

Q01102 (W42-A709)

Gene ID
Molecular Construction
N-term
P-selectin (W42-A709)
Accession # Q01102
Avi-6*His
C-term
Synonyms
CD62 antigen-like family member P; GMP-140; LECAM3; PADGEM
AA Sequence

WTYNYSTKAYSWNNSRVFCRRHFTDLVAIQNKNEIAHLNDVIPFFNSYYWIGIRKINNKWTWVGTNKTLTEEAENWADNEPNNKKNNQDCVEIYIKSNSAPGKWNDEPCFKRKRALCYTASCQDMSCSNQGECIETIGSYTCSCYPGFYGPECEYVKECGKVNIPQHVLMNCSHPLGEFSFNSQCTFSCAEGYELDGPGELQCLASGIWTNNPPKCDAVQCQSLEAPPHGTMACMHPIAAFAYDSSCKFECQPGYRARGSNTLHCTGSGQWSEPLPTCEAIACEPPEIPIHGSMDCVPSTGTFGYNSSCTFLCAEGFVLKGNDAIQCADSGQWTAPAPFCEALQCPEFPVPSKAQVNCSDPFGTLTYQSVCSFSCDEGSLLVGASVIRCLATGHWNGAPPECQAVSCAPMLSPENGSMTCVQPLGNSTYKSTCQFMCDEGFYLSGPERLDCSPSGHWTGTPPTCEAIKCPGIFAPEQGNLDCSHVHGEFGVGSICHFSCNEDFELLGSENVECTVSGRWSAPPPTCKGITSLPAPAVRCPALTTPGQGTMSCQHHLGSFGPNTTCYFGCKTGFTLRGANSLRCRASGQWTAVTPMCRAVKCSELHMDTAVAMNCSNPWGNFSYGSTCTFQCPEGQSLNGSVRATCREDGHWSDAMPTCQAGTLTIQEA

Molecular Weight

102-122 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His)
Cat. No.:
HY-P72408
Quantity:
MCE Japan Authorized Agent: