PACSIN2 Protein, Human (HEK293, His)
The PACSIN2 protein coordinates caveolar morphogenesis and endocytosis to dynamically regulate cell membrane structure. As a lipid-binding protein, PACSIN2 regulates phosphatidic acid-containing membranes. PACSIN2 Protein, Human (HEK293, His) is the recombinant human-derived PACSIN2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PACSIN2 protein coordinates caveolar morphogenesis and endocytosis to dynamically regulate cell membrane structure. As a lipid-binding protein, PACSIN2 regulates phosphatidic acid-containing membranes. PACSIN2 Protein, Human (HEK293, His) is the recombinant human-derived PACSIN2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
PACSIN2 protein plays a pivotal role in orchestrating the morphogenesis and endocytosis of caveolae, contributing to the dynamic regulation of cellular membrane structures. Functioning as a lipid-binding protein, PACSIN2 exhibits a particular affinity for phosphatidic acid-containing membranes, promoting their tubulation. Beyond its involvement in membrane shaping, PACSIN2 serves a crucial function in intracellular vesicle-mediated transport, facilitating the endocytosis of cell-surface receptors such as the EGF receptor, even in the absence of EGF stimulus. Furthermore, PACSIN2 demonstrates a multifaceted role in microbial infection, notably enhancing the efficiency of HIV-1 virion spread through cell-to-cell transfer and promoting protrusion engulfment during the cell-to-cell spread of bacterial pathogens like Listeria monocytogenes. Additionally, PACSIN2 contributes to lipid droplet formation, a process vital for HCV virion assembly. Overall, PACSIN2 emerges as a versatile regulator with significant implications in membrane dynamics, cellular transport, and infectious processes.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9UNF0-1 (M1-Q486)
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Molecular Construction
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N-term
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PACSIN2 (M1-Q486)
Accession # Q9UNF0-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
PACSIN2; Cytoplasmic Phosphoprotein PACSIN2; Protein Kinase C And Casein Kinase Substrate In Neurons 2; PACSIN2 Protein; SDPII; Syndapin II; Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2; Syndapin2; Syndapin-II; SdpII; Syndapin-2
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AA Sequence
MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCSDLMNCLHERARIEKAYAQQLTEWARRWRQLVEKGPQYGTVEKAWMAFMSEAERVSELHLEVKASLMNDDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRKAQKPWAKKLKEVEAAKKAHHAACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ
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Predicted Molecular Mass
56.7 kDa
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Molecular Weight
Approximately 88 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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