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PAK6 Protein, Human

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PAK6, a dynamic serine/threonine kinase, regulates gene transcription activated by AR or MAP2K6/MAPKK6. It phosphorylates and inhibits AR-mediated transcription, hindering ESR1-mediated transcription. PAK6 interacts with IQGAP1, suggesting a role in cytoskeleton regulation. Moreover, it protects cells from apoptosis by phosphorylating BAD, showcasing its multifaceted impact on transcriptional control and cytoskeletal dynamics. PAK6 Protein, Human is the recombinant human-derived PAK6 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

PAK6, a dynamic serine/threonine kinase, regulates gene transcription activated by AR or MAP2K6/MAPKK6. It phosphorylates and inhibits AR-mediated transcription, hindering ESR1-mediated transcription. PAK6 interacts with IQGAP1, suggesting a role in cytoskeleton regulation. Moreover, it protects cells from apoptosis by phosphorylating BAD, showcasing its multifaceted impact on transcriptional control and cytoskeletal dynamics. PAK6 Protein, Human is the recombinant human-derived PAK6 protein, expressed by E. coli , with tag free.

Background

PAK6, a serine/threonine protein kinase, serves as a regulator of gene transcription, with its kinase activity triggered by various effectors, including AR or MAP2K6/MAPKK6. Notably, it phosphorylates the DNA-binding domain of the androgen receptor/AR, hindering AR-mediated transcription, and also inhibits ESR1-mediated transcription. PAK6's involvement in cytoskeleton regulation is suggested by its interaction with IQGAP1. Additionally, PAK6 demonstrates a potential role in cell survival by protecting cells from apoptosis through the phosphorylation of BAD. This multifaceted kinase exhibits a regulatory impact on key cellular processes, showcasing its significance in transcriptional control and cytoskeletal dynamics.

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q9NQU5 (G383-Y674)

  • Gene ID
    106821730
  • Molecular Construction
    • N-term
    • PAK6 (G383-Y674)
      Accession # Q9NQU5
    • C-term
  • Protein Length

    Partial

  • Synonyms

    PAK6; P21(CDKN1A)-Activated Kinase 6; P21 (RAC1) Activated Kinase 6; Non-Specific Serine/Threonine Protein Kinase; PAK5; P21-Activated Kinase 6; P21 Protein (Cdc42/Rac)-Activated Kinase 6; PAK-6; Serine/Threonine-Protein Kinase PAK 6; PAK-5

  • AA Sequence

    GVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLY

  • Predicted Molecular Mass

    33.2 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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  • PAK6 Protein, Human Product Image