PCK2 Protein, Human (sf9, His-GST)

As a key enzyme in cellular metabolism, PCK2 protein catalyzes the conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), representing the rate-limiting step in the metabolic pathway and responsible for the production of glucose from other precursors derived from lactate and citric acid cycle. PCK2 Protein, Human (sf9, His-GST) is the recombinant human-derived PCK2 protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

Phosphoenolpyruvate carboxykinase 2 (PCK2) is an enzyme that plays a crucial role in glucose metabolism by catalyzing the rate-limiting step in the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP). This metabolic pathway is significant for the synthesis of glucose from lactate and other precursors derived from the citric acid cycle. PCK2's enzymatic activity contributes to gluconeogenesis, a process crucial for maintaining glucose homeostasis, especially during fasting or periods of increased energy demand. By converting OAA to PEP, PCK2 facilitates the generation of glucose, which is essential for providing energy to various tissues, including the brain and red blood cells. It has to emphasize PCK2's pivotal role in the metabolic pathway that enables the synthesis of glucose from non-carbohydrate sources, highlighting its importance in maintaining energy balance within the cell.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-His;N-GST

Q16822-1/NP_004554.3 (L33-M640)

5106  [NCBI]

Full Length of Isoform-1 Mature Protein

LSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.