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PCK2 Protein, Human (sf9, His-GST)

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As a key enzyme in cellular metabolism, PCK2 protein catalyzes the conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), representing the rate-limiting step in the metabolic pathway and responsible for the production of glucose from other precursors derived from lactate and citric acid cycle. PCK2 Protein, Human (sf9, His-GST) is the recombinant human-derived PCK2 protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

As a key enzyme in cellular metabolism, PCK2 protein catalyzes the conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), representing the rate-limiting step in the metabolic pathway and responsible for the production of glucose from other precursors derived from lactate and citric acid cycle. PCK2 Protein, Human (sf9, His-GST) is the recombinant human-derived PCK2 protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

Background

Phosphoenolpyruvate carboxykinase 2 (PCK2) is an enzyme that plays a crucial role in glucose metabolism by catalyzing the rate-limiting step in the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP). This metabolic pathway is significant for the synthesis of glucose from lactate and other precursors derived from the citric acid cycle. PCK2's enzymatic activity contributes to gluconeogenesis, a process crucial for maintaining glucose homeostasis, especially during fasting or periods of increased energy demand. By converting OAA to PEP, PCK2 facilitates the generation of glucose, which is essential for providing energy to various tissues, including the brain and red blood cells. It has to emphasize PCK2's pivotal role in the metabolic pathway that enables the synthesis of glucose from non-carbohydrate sources, highlighting its importance in maintaining energy balance within the cell.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His;N-GST
  • Accession

    Q16822-1/NP_004554.3 (L33-M640)

  • Gene ID
    5106  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • PCK2 (L33-M640)
      Accession # Q16822-1/NP_004554.3
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    PCK2; MtPCK2; Phosphoenolpyruvate Carboxykinase 2, Mitochondrial; Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); PEPCK2; Phosphoenolpyruvate Carboxykinase 2 Isoform 3; Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial; Epididymis Secretory Sper

  • AA Sequence

    LSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

  • Predicted Molecular Mass

    94.9 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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