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  5. PD-1 Protein, Canine (HEK293, His)

PD-1 Protein, Canine (HEK293, His)

Cat. No.: HY-P73342
COA Handling Instructions

The Angiopoietin-1 Protein is a secreted glycoprotein that is a key ligand in the vascular tyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells. PD-1 Protein, Canine (HEK293, His) is the recombinant canine-derived PD-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PD-1 Protein, Canine (HEK293, His) is 145 a.a., with molecular weight of ~34.88 and 65.02 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $145 In-stock
20 μg $235 In-stock
50 μg $440 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The Angiopoietin-1 Protein is a secreted glycoprotein that is a key ligand in the vascular tyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells. PD-1 Protein, Canine (HEK293, His) is the recombinant canine-derived PD-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PD-1 Protein, Canine (HEK293, His) is 145 a.a., with molecular weight of ~34.88 and 65.02 kDa.

Background

ANGPT1 encodes a secreted glycoprotein in the angiopoietin family and is a key ligand in the vasotyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. The Angiopoietin-1 Protein regulates the formation and stability of blood vessels and plays an important role in vascular development. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells[1][2][3].

Biological Activity

Measured by its ability to bind biotinylated human B7-H1-Fc in functional ELISA.

Species

Canine

Source

HEK293

Tag

C-His

Accession

XP_543338.3 (L25-L169)

Gene ID
Molecular Construction
N-term
PD-1 (L25-L169)
Accession # XP_543338.3
His
C-term
Synonyms
CD279; hPD-1; PDCD1; Programmed cell death 1; SLEB2
Molecular Weight

Approximately 34.88 and 65.02 kDa

Purity

Greater than 95% as determined by SEC-HPLC.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PD-1 Protein, Canine (HEK293, His)
Cat. No.:
HY-P73342
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