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  5. PD-L1 Protein, Mouse (Biotinylated, HEK293, His)

PD-L1 Protein, Mouse (Biotinylated, HEK293, His)

Cat. No.: HY-P77819
COA Handling Instructions

PD-L1 protein serves as a ligand for the inhibitory receptor PDCD1/PD-1 and is critical for maintaining immune tolerance. This interaction modulates T cell activation, limits effector responses and may stimulate IL10-producing T cell subsets. PD-L1 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived PD-L1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PD-L1 Protein, Mouse (Biotinylated, HEK293, His) is 220 a.a., with molecular weight of 45-60 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $250 In-stock
50 μg $510 In-stock
100 μg $850 In-stock
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  • Biological Activity

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Description

PD-L1 protein serves as a ligand for the inhibitory receptor PDCD1/PD-1 and is critical for maintaining immune tolerance. This interaction modulates T cell activation, limits effector responses and may stimulate IL10-producing T cell subsets. PD-L1 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived PD-L1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PD-L1 Protein, Mouse (Biotinylated, HEK293, His) is 220 a.a., with molecular weight of 45-60 kDa.

Background

PD-L1 protein plays a crucial role in maintaining immune tolerance to self by acting as a ligand for the inhibitory receptor PDCD1/PD-1. This interaction modulates the activation threshold of T-cells, limiting their effector response and potentially stimulating T-cell subsets that produce interleukin-10 (IL10). However, tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity and evade destruction by the immune system, thereby promoting tumor survival. The interaction between PD-L1 and PDCD1/PD-1 inhibits the function of cytotoxic T lymphocytes (CTLs), but blocking this pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, offering a promising strategy for cancer immunotherapy.

Biological Activity

Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-His antibody can bind Mouse PD-1, hFc Tag with an affinity constant of 0.31 μM as determined inSPR assay (Biacore T200)

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q9EP73 (F19-T238)

Gene ID
Molecular Construction
N-term
PD-L1 (F19-T238)
Accession # Q9EP73
His
C-term
Synonyms
CD274; PDL1; PD-L1; PD-L1B7 homolog 1; B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
AA Sequence

FTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQNRT

Molecular Weight

45-60 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS(pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PD-L1 Protein, Mouse (Biotinylated, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PD-L1 Protein, Mouse (Biotinylated, HEK293, His)
Cat. No.:
HY-P77819
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