PD-L2 Protein, Human (Biotinylated, HEK293, His-Avi)

PD-L2 Protein, operating independently of PDCD1, plays a vital role in T-cell proliferation and IFNG production. Its interaction with PDCD1 inhibits proliferation and cytokine production. The interplay between PD-L2 and PDCD1 regulates immune responses, influencing T-cell activation and function. This molecular interaction adds complexity to mechanisms governing T-cell behavior, highlighting PD-L2's versatile role in immune regulation. PD-L2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PD-L2 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

PD-L2 Protein is integral to the costimulatory signal crucial for T-cell proliferation and IFNG production, operating in a PDCD1-independent manner. Its interaction with PDCD1, however, functions to inhibit T-cell proliferation by impeding cell cycle progression and cytokine production. The intricate interplay between PD-L2 and PDCD1 underscores its role as a regulatory checkpoint in modulating immune responses, influencing the activation and function of T cells. This molecular interaction adds a layer of complexity to the dynamic mechanisms governing T-cell behavior, highlighting PD-L2's versatile role in immune regulation.

Human

HEK293

C-Avi;C-6*His

Q9BQ51-1 (L20-P219)

80380  [NCBI]

Extracellular Domain

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHP

38-60 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.