1. Recombinant Proteins
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins
  4. B7-DC/PD-L2 B7-DC/CD273
  5. PD-L2 Protein, Human (HEK293, Fc)

PD-L2 Protein, Human (HEK293, Fc)

Cat. No.: HY-P7399
COA Handling Instructions

PD-L2 Protein, Human (HEK293, Fc), a second ligand for PD-1, interacts with PD-1 negatively regulates T cell proliferation, cytokine production and cytotoxic activity.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
10 μg $90 In-stock
50 μg $190 In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References


PD-L2 Protein, Human (HEK293, Fc), a second ligand for PD-1, interacts with PD-1 negatively regulates T cell proliferation, cytokine production and cytotoxic activity.


Programmed death I (PD-I)-deficient mice develop a variety of autoimmune-like diseases, which suggests that this immunoinhibitory receptor plays an important role in tolerance. PD-1 ligand 2 (PD-L2) is a second ligand for PD-1. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4+ T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals. In contrast, at high antigen concentrations, PD-L2-PD-1 interactions reduce cytokine production but do not inhibit T cell proliferation. PD-L-PD-1 interactions lead to cell cycle arrest in G0/G1 but do not increase cell death. In addition, ligation of PD-1 + TCR leads to rapid phosphorylation of SHP-2, as compared to TCR ligation alone[1].

Biological Activity

5 µg/mL (100 µL/well) of immoblized recombinant human PD-L2-Fc can bind human Biotin-PD-1-Fc with a linear range of 2.44-39.06 ng/mL.








Q9BQ51 (L20-P219)

Gene ID
Molecular Construction
PD-L2 (L20-P219)
Accession # Q9BQ51
rHuPD-L2, Fc Chimera; Programmed cell death 1 ligand 2; B7DC; CD273
AA Sequence


Molecular Weight

Approximately 75 kDa


Greater than 95% as determined by reducing SDS-PAGE.


Lyophilized powder.


Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.


It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


Room temperature in continental US; may vary elsewhere.

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name



Applicant Name *


Email Address *

Phone Number *


Organization Name *

Department *


Requested quantity *

Country or Region *



Bulk Inquiry

Inquiry Information

Product Name:
PD-L2 Protein, Human (HEK293, Fc)
Cat. No.:
MCE Japan Authorized Agent: