PDE9A Protein, Human (sf9, His-GST)
PDE9A is a phosphodiesterase that uniquely hydrolyzes second messengers with unparalleled specificity, particularly in the regulation of natriuretic peptide-dependent signaling in the heart, which is critical for the control of cardiac hypertrophy. Its specificity rules out effects on nitric oxide-dependent signaling in the heart. PDE9A Protein, Human (sf9, His-GST) is the recombinant human-derived PDE9A protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PDE9A is a phosphodiesterase that uniquely hydrolyzes second messengers with unparalleled specificity, particularly in the regulation of natriuretic peptide-dependent signaling in the heart, which is critical for the control of cardiac hypertrophy. Its specificity rules out effects on nitric oxide-dependent signaling in the heart. PDE9A Protein, Human (sf9, His-GST) is the recombinant human-derived PDE9A protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
PDE9A, a phosphodiesterase enzyme, exhibits remarkable specificity by hydrolyzing the second messenger cwith the highest affinity and selectivity compared to other members of the cyclic nucleotide phosphodiesterase family. Its distinctive role is evident in regulating natriuretic-peptide-dependent csignaling in the heart, where it serves as a key regulator of cardiac hypertrophy in myocytes and muscle. Notably, PDE9A does not influence nitric oxide-dependent csignaling in the heart, highlighting its specificity for natriuretic-peptide-dependent pathways. Further investigations are warranted to determine whether its role in hydrolyzing natriuretic-peptide-dependent cis exclusive to the heart or represents a broader feature of the protein. Additionally, in the brain, PDE9A contributes to cognitive functions such as learning and long-term memory, emphasizing its diverse physiological roles in different tissues.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-His;N-GST
-
Accession
O76083-2 (M1-A533)
-
Molecular Construction
-
N-term
-
His-GST
-
PDE9A (M1-A533)
Accession # O76083-2 -
C-term
-
-
Protein Length
Full Length of Isoform-2
-
Synonyms
PDE9A; Phosphodiesterase PDE9A21; Phosphodiesterase 9A; Phosphodiesterase; High Affinity CGMP-Specific 3',5'-Cyclic Phosphodiesterase 9A; HSPDE9A2; CGMP-Specific 3',5'-Cyclic Phosphodiesterase Type 9
-
AA Sequence
MGSGSSSYRPKAIYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAIKQLSEREELIQSVLAQVAEQFSRAFKINELKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIKKMREELAARSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFSQTDILILMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAVAFQILAEPECNIFSNIPPDGFKQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHMTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPMVEEIMLQPLWESRDRYEELKRIDDAMKELQKKTDSLTSGATEKSRERSRDVKNSEGDCA
-
Predicted Molecular Mass
89.5 kDa
-
Molecular Weight
Approximately 75 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
