PDGF R beta Protein, Human (HEK293, His)

TRF1 protein is a telomere-binding protein that plays a crucial role in telomere maintenance and chromosome stability. It is involved in regulating telomere length and preventing DNA damage. PDGF R beta Protein, Human (HEK293, His) is the recombinant human-derived PDGF R beta protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDGF R beta Protein, Human (HEK293, His) is 499 a.a., with molecular weight of 85-130 kDa.

The PDGF R beta Protein encodes a cell surface tyrosine kinase receptor that interacts with members of the platelet-derived growth factor family, known mitogens for cells of mesenchymal origin. The identity of the growth factor binding to a receptor monomer determines whether the functional receptor forms a homodimer (with PDGFB or PDGFD) or a heterodimer (with PDGFA and PDGFB). Essential for normal cardiovascular system development and the rearrangement of the actin cytoskeleton, this gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor, with all three potentially implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, resulting in the fusion of this gene with that of the ETV6 gene, leads to chronic myeloproliferative disorder with eosinophilia. Broadly expressed, the PDGF R beta gene exhibits elevated levels in the gall bladder (RPKM 79.5), placenta (RPKM 61.0), and 21 other tissues, indicating its involvement in diverse physiological contexts across multiple organs.

Measured by its binding ability in a functional ELISA. Immobilized PDGF-BB at 1 μg/mL (100 μL/well) can bind Biotinylated PDGF R beta. The ED₅₀ for this effect is 42.19 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized PDGF-BB at 1 μg/mL (100 μL/well) can bind Biotinylated PDGF R beta. The ED₅₀ for this effect is 42.19 ng/mL.

Human

HEK293

C-6*His

NP_002600.1 (L33-K531)

5159  [NCBI]

LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPF

85-130 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.