Pepsinogen C/PGC Protein, Human (HEK293, His)

Pepsinogen C/PGC protein hydrolyzes various proteins, playing a crucial role in digestion by breaking them down into smaller peptides. Its proficiency in protein hydrolysis contributes to the initial stages of digestion, making it vital in the overall digestive cascade. Pepsinogen C/PGC Protein, Human (HEK293, His) is the recombinant human-derived Pepsinogen C/PGC protein, expressed by HEK293 , with C-His labeled tag. The total length of Pepsinogen C/PGC Protein, Human (HEK293, His) is 372 a.a., with molecular weight of ~41 kDa.

Pepsinogen C, also known as PGC protein, serves as a key enzyme with the ability to hydrolyze a diverse range of proteins. This enzymatic activity underscores its crucial role in the digestive process, as it participates in breaking down proteins into smaller peptides. PGC's proficiency in protein hydrolysis contributes significantly to the initial stages of digestion, playing a vital role in the overall digestive cascade.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is 1431.763 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P20142-1 (A17-A388)

5225  [NCBI]

AVVKVPLKKFKSIRETMKEKGLLGEFLRTHKYDPAWKYRFGDLSVTYEPMAYMDAAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVFSVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFATAA

Approximately 41 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.