Pepsinogen C/PGC Protein, Human (HEK293, His)
Based on 1 Customer Validation
Pepsinogen C/PGC protein hydrolyzes various proteins, playing a crucial role in digestion by breaking them down into smaller peptides. Its proficiency in protein hydrolysis contributes to the initial stages of digestion, making it vital in the overall digestive cascade. Pepsinogen C/PGC Protein, Human (HEK293, His) is the recombinant human-derived Pepsinogen C/PGC protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Pepsinogen C/PGC protein hydrolyzes various proteins, playing a crucial role in digestion by breaking them down into smaller peptides. Its proficiency in protein hydrolysis contributes to the initial stages of digestion, making it vital in the overall digestive cascade. Pepsinogen C/PGC Protein, Human (HEK293, His) is the recombinant human-derived Pepsinogen C/PGC protein, expressed by HEK293 , with C-His labeled tag.
Background
Pepsinogen C, also known as PGC protein, serves as a key enzyme with the ability to hydrolyze a diverse range of proteins. This enzymatic activity underscores its crucial role in the digestive process, as it participates in breaking down proteins into smaller peptides. PGC's proficiency in protein hydrolysis contributes significantly to the initial stages of digestion, playing a vital role in the overall digestive cascade.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is 1431.763 pmol/min/µg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P20142-1/NP_002621.1 (A17-A388)
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Molecular Construction
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N-term
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PGC (A17-A388)
Accession # P20142-1/NP_002621.1 -
His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
PGC; Pepsinogen Group II; Progastricsin; Preprogastricsin; Pepsinogen C; Pepsin C; Gastricsin; PEPC; Progastricsin (Pepsinogen C); PGII
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AA Sequence
AVVKVPLKKFKSIRETMKEKGLLGEFLRTHKYDPAWKYRFGDLSVTYEPMAYMDAAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVFSVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFATAA
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Molecular Weight
Approximately 41 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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