Periostin/OSF-2 Protein, Rat (HEK293, His)

Periostin/OSF-2 Protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin/OSF-2 Protein, Rat (HEK293, His) is the recombinant rat-derived Periostin/OSF-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Periostin/OSF-2 Protein, Rat (HEK293, His) is 777 a.a., with molecular weight of approximately 95 kDa.

Periostin, a multifunctional molecule, plays a pivotal role in cellular processes by inducing cell attachment and spreading, demonstrating its significance in cell adhesion dynamics. Moreover, Periostin contributes to the structural integrity of connective tissues by enhancing the incorporation of BMP1 into the fibronectin matrix, leading to the subsequent proteolytic activation of lysyl oxidase LOX. Structurally, Periostin exists as a homodimer, and functionally, it interacts with key partners including BMP1 and fibronectin. This intricate network of interactions underscores the diverse functions of Periostin in mediating cellular adhesion and contributing to the extracellular matrix architecture of connective tissues, highlighting its essential role in maintaining tissue integrity and function^[1][2].

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 44.48 ng/mL, corresponding to a specific activity is 2.248×10⁴ units/mg.

Rat

HEK293

C-6*His

D3ZAF5 (N24-S800)

361945  [NCBI]

NSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWDNLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDELSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHEKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTNEEREILIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYVKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYTTKIITKLVEPKIKVIQGSLQPIIKTEGPAMTKIHIEGEPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLQKFLQKDTPAKKIQANKRVQGS

approximately 95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.