Copy Link Print Information

Periostin/OSF-2 Protein, Rat (HEK293, His)

Customer Review

Based on 1 Customer Validation

Periostin/OSF-2 Protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin/OSF-2 Protein, Rat (HEK293, His) is the recombinant rat-derived Periostin/OSF-2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Periostin/OSF-2 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Periostin/OSF-2 Protein, Rat (HEK293, His)
  • Periostin/OSF-2 Protein, Rat (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Periostin/OSF-2 Protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin/OSF-2 Protein, Rat (HEK293, His) is the recombinant rat-derived Periostin/OSF-2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Periostin, a multifunctional molecule, plays a pivotal role in cellular processes by inducing cell attachment and spreading, demonstrating its significance in cell adhesion dynamics. Moreover, Periostin contributes to the structural integrity of connective tissues by enhancing the incorporation of BMP1 into the fibronectin matrix, leading to the subsequent proteolytic activation of lysyl oxidase LOX. Structurally, Periostin exists as a homodimer, and functionally, it interacts with key partners including BMP1 and fibronectin. This intricate network of interactions underscores the diverse functions of Periostin in mediating cellular adhesion and contributing to the extracellular matrix architecture of connective tissues, highlighting its essential role in maintaining tissue integrity and function[1][2].

Verified Bioactivity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 44.48 ng/mL, corresponding to a specific activity is 2.248×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 44.48 ng/mL, corresponding to a specific activity is 2.248×104 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-6*His
  • Accession

    D3ZAF5 (N24-S800)

  • Gene ID
    361945  [NCBI]
  • Molecular Construction
    • N-term
    • Periostin (N24-S800)
      Accession # D3ZAF5
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    POSTN; Osteoblast Specific Factor 2 (Fasciclin I-Like); Periostin; Periodontal Ligament-Specific Periostin; OSF-2; Osteoblast Specific Factor 2; PN; Osteoblast-Specific Factor 2; Periostin, Osteoblast Specific Factor; PDLPOSTN; OSF2

  • AA Sequence

    NSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWDNLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDELSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHEKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTNEEREILIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYVKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYTTKIITKLVEPKIKVIQGSLQPIIKTEGPAMTKIHIEGEPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLQKFLQKDTPAKKIQANKRVQGS

  • Molecular Weight

    Approximately 77-95 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 44.48 ng/mL, corresponding to a specific activity is 2.248×104 units/mg.

  • Periostin/OSF-2 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Periostin/OSF-2 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Periostin/OSF-2 Protein, Rat (HEK293, His)
  • Periostin/OSF-2 Protein, Rat (HEK293, His) Product Image