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Periostin Protein, Mouse (Q62009-2, HEK293, His)

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Periostin protein is a multifunctional molecule that plays a key role in cellular processes by inducing cell attachment and spreading, emphasizing its importance in cell adhesion dynamics. Furthermore, Periostin contributes to the structural integrity of connective tissue by enhancing the binding of BMP1 to the fibronectin matrix and promoting subsequent proteolytic activation of lysyl oxidase LOX. Periostin Protein, Mouse (Q62009-2, HEK293, His) is the recombinant mouse-derived Periostin protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Periostin Protein, Mouse (Q62009-2, HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Periostin protein is a multifunctional molecule that plays a key role in cellular processes by inducing cell attachment and spreading, emphasizing its importance in cell adhesion dynamics. Furthermore, Periostin contributes to the structural integrity of connective tissue by enhancing the binding of BMP1 to the fibronectin matrix and promoting subsequent proteolytic activation of lysyl oxidase LOX. Periostin Protein, Mouse (Q62009-2, HEK293, His) is the recombinant mouse-derived Periostin protein, expressed by HEK293 , with C-His labeled tag.

Background

Periostin protein, a multifunctional molecule, plays a pivotal role in cellular processes by inducing cell attachment and spreading, demonstrating its significance in cell adhesion dynamics. Moreover, Periostin contributes to the structural integrity of connective tissues by enhancing the incorporation of BMP1 into the fibronectin matrix, leading to the subsequent proteolytic activation of lysyl oxidase LOX. Structurally, Periostin exists as a homodimer, and functionally, it interacts with key partners including BMP1 and fibronectin. This intricate network of interactions underscores the diverse functions of Periostin in mediating cellular adhesion and contributing to the extracellular matrix architecture of connective tissues, highlighting its essential role in maintaining tissue integrity and function.

Verified Bioactivity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10 μg/mL, 100 μL/well), approximately >40% will adhere specifically after 30 minutes at 37°C.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q62009-2/NP_056599.1 (N24-Q811)

  • Gene ID
    50706  [NCBI]
  • Molecular Construction
    • N-term
    • Periostin (N24-Q811)
      Accession # Q62009-2/NP_056599.1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    POSTN; Osteoblast Specific Factor 2 (Fasciclin I-Like); Periostin; Periodontal Ligament-Specific Periostin; OSF-2; Osteoblast Specific Factor 2; PN; Osteoblast-Specific Factor 2; Periostin, Osteoblast Specific Factor; PDLPOSTN; OSF2

  • AA Sequence

    NSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWENLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFLEAEDDLSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHDKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTSEERELLIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYLKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYRPAMTKIQIEGDPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKQTREERIITGPEIKYTRISTGGGETGETLQKFLQKEVSKVTKFIEGGDGHLFEDEEIKRLLQGDTPAKKIPANKRVQGPRRRSREGRSQ

  • Predicted Molecular Mass

    89 kDa

  • Molecular Weight

    Approximately 80-85 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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