PLA2G2A Protein, Human (HEK293, His, solution)

The PLA2G2A protein is a secreted calcium-dependent phospholipase A2 that selectively targets extracellular phospholipids and contributes to host antimicrobial defense, inflammation, and tissue regeneration. It has phospholipase A2 activity, which preferentially hydrolyzes fatty acyl groups, phosphatidylethanolamine and phosphatidylglycerol. PLA2G2A Protein, Human (HEK293, His) is the recombinant human-derived PLA2G2A protein, expressed by HEK293 , with C-His labeled tag.

PLA2G2A, a secretory calcium-dependent phospholipase A2, selectively targets extracellular phospholipids and holds significant implications in host antimicrobial defense, inflammatory response, and tissue regeneration. Operating with phospholipase A2 activity, it hydrolyzes the ester bond of the fatty acyl group at the sn-2 position of phospholipids, displaying a preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. PLA2G2A actively contributes to lipid remodeling in cellular membranes and the generation of lipid mediators crucial for pathogen clearance, exerting bactericidal activity against Gram-positive bacteria. During sterile inflammation, it targets membrane phospholipids of extracellular mitochondria, releasing free unsaturated fatty acids like arachidonate. This arachidonate is utilized by neighboring leukocytes for synthesizing inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, PLA2G2A promotes the release of potent damage-associated molecular pattern molecules into circulation, activating the innate immune response. In the intestinal crypt, PLA2G2A serves as a stem cell regulator, mediating Paneth cell differentiation and supporting stem cell functions by inhibiting the Wnt signaling pathway. Secreted in the intestinal lumen during inflammation, it acts in an autocrine manner, promoting prostaglandin E2 synthesis that stimulates the Wnt signaling pathway in intestinal stem cells and contributes to tissue regeneration. Additionally, PLA2G2A may play a role in the biosynthesis of N-acyl ethanolamines, regulating energy metabolism and inflammation, and it exhibits integrin-dependent cell proliferation by binding to and activating integrins ITGAV:ITGB3, ITGA4:ITGB1, and ITGA5:ITGB1 through distinct ligand-binding sites.

Human

HEK293

C-His

P14555/NP_000291.1 (N21-C144)

5320

Full Length of Mature Protein

NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC

Approximately 19 kDa, based on SDS-PAGE under reducing condition, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.