PLAU/uPA Protein, Rat (HEK293, His)

PLAU/uPA proteins lack conserved residues critical for feature annotation propagation, suggesting potential differences in molecular interactions in plasminogen activation and fibrinolysis regulation. PLAU/uPA Protein, Rat (HEK293, His) is the recombinant rat-derived PLAU/uPA protein, expressed by HEK293 , with C-His labeled tag.

PLAU, also known as uPA (urokinase-type plasminogen activator), is characterized by the absence of conserved residues required for feature annotation propagation. This unique feature raises questions about the structure and function of PLAU, suggesting potential distinctions in its molecular interactions in the context of plasminogen activation and fibrinolysis regulation. The absence of these conserved residues emphasizes the specific properties of PLAU, which warrants in-depth studies to elucidate its unique role in cellular processes and pathological conditions.

Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Rat PLAU, His Tag with an affinity constant of 4.31 μM as determined in SPR assay (Biacore T200).

Rat

HEK293

C-His

Q3KR76/NP_037217.3 (G20-F432)

25619  [NCBI]

Full Length of Mature Peptide

GSELGASDESNCGCQNGGVCVSYKYFSSIRRCSCPKKFKGEHCEIDTSKTCYHGNGQSYRGKANTDTKGRPCLAWNSPAVLQQTYNAHRSDALSLGLGKHNYCRNPDNQRRPWCYVQIGLKQFVQECMVQDCSLSKKPSSTVDQQGFQCGQKALRPRFKIVGGEFTVVENQPWFAAIYLKNKGGSPPSFKCGGSLISPCWVASATHCFVNQPKKEEYVVYLGQSKRNSYNPGEMKFEVEQLILHEDFSDETLAFHNDIALLKIRTSTGQCAQPSRTIQTICLPPRFGDAPFGSDCEITGFGQESATDYFYPKDLKMSVVKIISHEQCKQPHYYGSEINYKMLCAADPEWKTDSCSGDSGGPLICNIDGRPTLSGIVSWGSGCAEKNKPGVYTRVSYFLNWIQSHIGEENGLAF

30-35 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.