PPC-DC Protein, Human (His)

PPC-DC proteins are central players in cellular ubiquitination, covalently linking ubiquitin to target proteins. Its critical role extends to the selective degradation of misfolded membrane proteins, an important process in endoplasmic reticulum-associated degradation (ERAD). PPC-DC Protein, Human (His) is the recombinant human-derived PPC-DC protein, expressed by E. coli , with N-6*His labeled tag. The total length of PPC-DC Protein, Human (His) is 204 a.a., with molecular weight of 25-27 kDa.

UBE2J2 protein serves as a key player in cellular ubiquitination processes, facilitating the covalent attachment of ubiquitin to target proteins. Its functional significance extends to the selective degradation of misfolded membrane proteins, a crucial aspect of endoplasmic reticulum-associated degradation (ERAD). This involvement underscores UBE2J2's role in maintaining cellular homeostasis by actively participating in the quality control of protein folding within the endoplasmic reticulum. Additionally, in collaboration with the GATOR2 complex, UBE2J2 catalyzes the 'Lys-6'-linked ubiquitination of NPRL2, further emphasizing its regulatory influence on specific cellular pathways, particularly those related to protein quality control.

Data is not available.

Human

E. coli

N-6*His

Q96CD2 (M1-S204)

60490  [NCBI]

MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS

25-27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.