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  4. Prostasin/PRSS8 Protein, Rat (HEK293, His)

Prostasin/PRSS8 Protein, Rat (HEK293, His)

Cat. No.: HY-P77155
COA Handling Instructions

Prostasin/PRSS8 Protein, featuring trypsin-like cleavage specificity, crucially activates the epithelial sodium channel (ENaC) by cleaving gamma subunits (SCNN1G) and modulating ion transport. Structurally, it forms a disulfide bond-linked heterodimer with light and heavy chains, highlighting its intricate role in cellular responses. Prostasin/PRSS8 Protein, Rat (HEK293, His) is the recombinant rat-derived Prostasin/PRSS8 protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostasin/PRSS8 Protein, Rat (HEK293, His) is 293 a.a., with molecular weight of ~32.9 KDa.

For research use only. We do not sell to patients.

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Description

Prostasin/PRSS8 Protein, featuring trypsin-like cleavage specificity, crucially activates the epithelial sodium channel (ENaC) by cleaving gamma subunits (SCNN1G) and modulating ion transport. Structurally, it forms a disulfide bond-linked heterodimer with light and heavy chains, highlighting its intricate role in cellular responses. Prostasin/PRSS8 Protein, Rat (HEK293, His) is the recombinant rat-derived Prostasin/PRSS8 protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostasin/PRSS8 Protein, Rat (HEK293, His) is 293 a.a., with molecular weight of ~32.9 KDa.

Background

Prostasin/PRSS8, characterized by a trypsin-like cleavage specificity with a preference for poly-basic substrates, plays a crucial role in stimulating the activity of the epithelial sodium channel (ENaC). Its activation involves the cleavage of the gamma subunits (SCNN1G) to modulate ion transport processes. Structurally, Prostasin/PRSS8 forms a heterodimer comprising light and heavy chains, united by a disulfide bond, underscoring its functional complexity in mediating cellular responses.

Biological Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The specific activity is 31.804 pmoL/min/µg, as measured under the described conditions.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q9ES87 (A30-R322)

Gene ID

192107  [NCBI]

Molecular Construction
N-term
Prostasin (A30-R322)
Accession # Q9ES87
His
C-term
Synonyms
Channel-activating protease 1; CAP1; Serine protease 8
AA Sequence

ADGTEASCGAVIQPRITGGGSAKPGQWPWQVSITYNGVHVCGGSLVSNQWVVSAAHCFPREHSKEEYEVKLGAHQLDSFSNDIVVHTVAQIISHSSYREEGSQGDIALIRLSSPVTFSRYIRPICLPAANASFPNGLHCTVTGWGHVAPSVSLQTPRPLQQLEVPLISRETCSCLYNINAVPEEPHTIQQDMLCAGYVKGGKDACQGDSGGPLSCPIDGLWYLAGIVSWGDACGAPNRPGVYTLTSTYASWIHHHVAELQPRVVPQTQESQPDGHLCNHHPVFNLAAAQKLSR

Molecular Weight

Approximately 32.9 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Prostasin/PRSS8 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Prostasin/PRSS8 Protein, Rat (HEK293, His)
Cat. No.:
HY-P77155
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