2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His)

Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His)

Cat. No.: HY-P700620
Handling Instructions Technical Support

Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
Background

Prostatic acid phosphatase (ACPP) is a non-specific tyrosine phosphatase that operates under acidic conditions (pH 4-6), demonstrating its versatility in dephosphorylating a diverse array of substrates, including alkyl, aryl, and acyl orthophosphate monoesters, as well as phosphorylated proteins. Notably, ACPP exhibits lipid phosphatase activity, contributing to the inactivation of lysophosphatidic acid in seminal plasma. Functioning as a tumor suppressor in prostate cancer, ACPP plays a crucial role in dephosphorylating ERBB2 and deactivating MAPK-mediated signaling, thereby exerting control over cellular processes implicated in cancer progression. Beyond its tyrosine phosphatase activity, ACPP showcases ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP. This additional function suggests a role in pain modulation, where adenosine acts as a pain suppressor, emphasizing the multifaceted nature of ACPP in cellular processes.
Species

Human
Source

HEK293
Tag

N-10*His
Accession

P15309-2 (K33-I418)
Gene ID

55  [NCBI]
Molecular Construction
N-term
10*His
ACPP (K33-I418)
Accession # P15309-2
C-term
Protein Length

Full Length of Isoform-2 Mature Protein
Synonyms
ACP-3; ACPP; Prostatic Acid Phosphatase; PAPf39; PAP
AA Sequence

KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLKVIFAVAFCLISAVLMVLLFIHIRRGLCWQRESYGNI

Predicted Molecular Mass
47.5 kDa
Glycosylation
Yes
Purity

Purity analysis by SDS-PAGE is not available for VLP proteins.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His)

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His)
Cat. No.:
HY-P700620
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.