Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His)
Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Prostatic acid Phosphatase/ACPP Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
Background
Prostatic acid phosphatase (ACPP) is a non-specific tyrosine phosphatase that operates under acidic conditions (pH 4-6), demonstrating its versatility in dephosphorylating a diverse array of substrates, including alkyl, aryl, and acyl orthophosphate monoesters, as well as phosphorylated proteins. Notably, ACPP exhibits lipid phosphatase activity, contributing to the inactivation of lysophosphatidic acid in seminal plasma. Functioning as a tumor suppressor in prostate cancer, ACPP plays a crucial role in dephosphorylating ERBB2 and deactivating MAPK-mediated signaling, thereby exerting control over cellular processes implicated in cancer progression. Beyond its tyrosine phosphatase activity, ACPP showcases ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP. This additional function suggests a role in pain modulation, where adenosine acts as a pain suppressor, emphasizing the multifaceted nature of ACPP in cellular processes.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-10*His
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Accession
P15309-2 (K33-I418)
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Molecular Construction
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N-term
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10*His
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ACPP (K33-I418)
Accession # P15309-2 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
ACP3; Acid Phosphatase, Prostate; Prev. ACPP; Ecto-5'-Nucleotidase; Prostatic Acid Phosphatase; TMPase; TM-PAP; 5'-NT; ACP-3; Prostatic Acid Phosphotase; PAP; Acid Phosphatase; Protein Tyrosine Phosphatase ACP3; 5'-Nucleotidase; Acid Phosphatase 3; Thiami
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AA Sequence
KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLKVIFAVAFCLISAVLMVLLFIHIRRGLCWQRESYGNI
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Predicted Molecular Mass
47.5 kDa
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Glycosylation
Yes
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Purity
Purity analysis by SDS-PAGE is not available for VLP proteins.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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