SHP-2 Protein, Human (T253M, Q257L, His)

SHP-2 protein, a key player downstream of receptor and cytoplasmic protein tyrosine kinases, facilitates signal transduction from the cell surface to the nucleus. It positively regulates the MAPK pathway and dephosphorylates critical substrates, including GAB1, ARHGAP35, EGFR, ROCK2 ('Tyr-722'), and CDC73. SHP-2's diverse functions extend to dephosphorylating SOX9, inhibiting its activity, and promoting ossification, along with targeting tyrosine-phosphorylated NEDD9/CAS-L to modulate various cellular signaling cascades. SHP-2 Protein, Human (T253M, Q257L, His) is the recombinant human-derived SHP-2 protein, expressed by E. coli , with N-6*His labeled tag and T253M, Q257L mutation. The total length of SHP-2 Protein, Human (T253M, Q257L, His) is 592 a.a., with molecular weight of 72 kDa.

SHP-2 protein functions downstream of various receptor and cytoplasmic protein tyrosine kinases, participating in signal transduction from the cell surface to the nucleus. It positively regulates the MAPK signal transduction pathway and plays a crucial role in dephosphorylating key substrates, including GAB1, ARHGAP35, EGFR, ROCK2 (at 'Tyr-722'), and CDC73. Additionally, SHP-2 dephosphorylates SOX9 on tyrosine residues, leading to the inactivation of SOX9 and the promotion of ossification. Furthermore, it targets tyrosine-phosphorylated NEDD9/CAS-L for dephosphorylation, highlighting its diverse roles in modulating cellular signaling cascades.

Human

E. coli

N-6*His

Q06124 (T2-R593, T253M, Q257L)

5781  [NCBI]

TSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFEMLQQLECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

72 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 40 mM Tris, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.