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SHP-2 Protein, Human (T253M, Q257L, His)

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The SHP-2 protein is a key player downstream of the receptor and cytoplasmic protein tyrosine kinases, facilitating signal transduction from the cell surface to the nucleus. It actively regulates the MAPK pathway and dephosphorylates key substrates, including GAB1, ARHGAP35, EGFR, ROCK2 ("Tyr-722"), and CDC73. SHP-2 Protein, Human (T253M, Q257L, His) is the recombinant human-derived SHP-2 protein, expressed by E. coli , with N-6*His labeled tag and T253M, Q257L mutation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SHP-2 Protein, Human (T253M, Q257L, His)
  • SHP-2 Protein, Human (T253M, Q257L, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The SHP-2 protein is a key player downstream of the receptor and cytoplasmic protein tyrosine kinases, facilitating signal transduction from the cell surface to the nucleus. It actively regulates the MAPK pathway and dephosphorylates key substrates, including GAB1, ARHGAP35, EGFR, ROCK2 ("Tyr-722"), and CDC73. SHP-2 Protein, Human (T253M, Q257L, His) is the recombinant human-derived SHP-2 protein, expressed by E. coli , with N-6*His labeled tag and T253M, Q257L mutation.

Background

SHP-2 protein functions downstream of various receptor and cytoplasmic protein tyrosine kinases, participating in signal transduction from the cell surface to the nucleus. It positively regulates the MAPK signal transduction pathway and plays a crucial role in dephosphorylating key substrates, including GAB1, ARHGAP35, EGFR, ROCK2 (at 'Tyr-722'), and CDC73. Additionally, SHP-2 dephosphorylates SOX9 on tyrosine residues, leading to the inactivation of SOX9 and the promotion of ossification. Furthermore, it targets tyrosine-phosphorylated NEDD9/CAS-L for dephosphorylation, highlighting its diverse roles in modulating cellular signaling cascades.

Verified Bioactivity

Measured by its ability to dephosphorylate a tyrosine residue in a peptide EGF Receptor Substrate 2 (HY-P0320, Phospho-Tyr5 (TFA)) that incubate at 37°C for 20 min. The specific activity is 3.01 μmol/min/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q06124-2 (T2-R593, T253M, Q257L)

  • Gene ID
    5781  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • SHP-2 (T2-R593, T253M, Q257L)
      Accession # Q06124
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    PTPN11; Protein Tyrosine Phosphatase Non-Receptor Type 11 Isoform 1; Prev. NS1; Tyrosine-Protein Phosphatase Non-Receptor Type; SH-PTP2; Protein-Tyrosine-Phosphatase; PTP2C; Noonan Syndrome 1; BPTP3; CDNA, FLJ92431; SHP-2; PTPN11 Protein; SHP2; METCDS; SH

  • AA Sequence

    TSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFEMLQQLECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

  • Molecular Weight

    Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 40 mM Tris, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 6% Trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • SHP-2 Protein, Human (T253M, Q257L, His) Product Image
    1/16
  • Experimental Validation Results for SHP-2 Protein, Human (T253M, Q257L, His)
  • SHP-2 Protein, Human (T253M, Q257L, His) Product Image