Ribonuclease UK114/HRSP12 Protein, Human (His)

The ribonuclease UK114/HRSP12 protein catalyzes the hydrolytic deamination of enamine/imine intermediates, reducing the potential toxicity of these reactive metabolites by releasing ammonia. Ribonuclease UK114/HRSP12 Protein, Human (His) is the recombinant human-derived Ribonuclease UK114/HRSP12 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Ribonuclease UK114/HRSP12 Protein, Human (His) is 137 a.a., with molecular weight of 14-16 kDa.

Ribonuclease UK114/HRSP12 protein plays a crucial role in cellular metabolism by catalyzing the hydrolytic deamination of enamine/imine intermediates that arise during normal metabolic processes. This enzymatic activity is thought to facilitate the safe release of ammonia from these potentially toxic reactive metabolites, thereby mitigating their impact on cellular components. HRSP12 is particularly associated with enamine/imine intermediates formed by various pyridoxal-5'-phosphate-dependent dehydratases, including L-threonine dehydratase. Moreover, it contributes to endoribonucleolytic cleavage of specific transcripts by promoting the recruitment of the ribonuclease P/MRP complex. Acting as a bridge between YTHDF2 and the ribonuclease P/MRP complex, HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs featuring a 5'-GGUUC-3' motif. The cooperative binding of HRSP12 and YTHDF2 to such transcripts leads to the recruitment of the ribonuclease P/MRP complex, facilitating subsequent endoribonucleolytic cleavage.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P52758 (M1-L137)

10247  [NCBI]

MSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL

14-16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.