RIPK1 Protein, Human (sf9, His, GST)

RIPK1 protein is a serine-threonine kinase that centrally regulates TNF-mediated apoptosis, necroptosis, and inflammatory pathways. Functionally, it plays a kinase-dependent role in the regulation of cell death and a kinase-independent scaffolding function in the control of inflammatory signaling and cell survival. RIPK1 Protein, Human (sf9, His, GST) is the recombinant human-derived RIPK1 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

RIPK1, a serine-threonine kinase, stands as a pivotal regulator orchestrating TNF-mediated apoptosis, necroptosis, and inflammatory pathways. Functionally, RIPK1 exhibits kinase-dependent roles in cell death regulation and kinase-independent scaffold functions governing inflammatory signaling and cell survival. As a scaffold protein within the TNF-R1 signaling complex, RIPK1 promotes cell survival by activating the canonical NF-kappa-B pathway. In the context of cell death, RIPK1, through its kinase activity, crucially regulates the assembly of death-inducing complexes—complex IIa (RIPK1-FADD-CASP8) for apoptosis and complex IIb (RIPK1-RIPK3-MLKL) for necroptosis. In normal conditions, RIPK1 inhibits RIPK3-dependent necroptosis by impeding the interaction of TRADD with FADD, thus limiting aberrant CASP8 activation. Additionally, RIPK1 contributes to the inflammatory response by fostering the transcriptional production of pro-inflammatory cytokines like interleukin-6 (IL6). Notably, RIPK1's kinase activity extends to phosphorylating RIPK3, DAB2IP, and participating in ZBP1-induced NF-kappa-B activation in response to DNA damage, highlighting its multifaceted roles in cellular processes.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-8*His;N-GST

Q13546-1 (M1-A327)

8737

Partial

MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVA

Approximately 60 KDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 20% glycerol, 5 mM DTT, 0.1 M trehalose.
2.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT, 0.1 M trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.