RIPK1 Protein, Mouse (C-His)
Based on 1 Customer Validation
RIPK1 Protein, Mouse (C-His) is the recombinant mouse-derived RIPK1 protein, expressed by E. coli, with C-6*His tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
RIPK1 Protein, Mouse (C-His) is the recombinant mouse-derived RIPK1 protein, expressed by E. coli, with C-6*His tag.
p-RIPK1 is a serine-threonine kinase that serves as a key regulator of TNF-mediated apoptosis, necroptosis, and inflammatory pathways. It exhibits dual kinase-dependent and -independent functions: its kinase activity governs cell death (e.g., by forming complex IIa for apoptosis and complex IIb for necroptosis), while its scaffold role promotes cell survival via recruitment to the TNF-R1 signaling complex (TNF-RSC/complex I) and activation of the canonical NF-kappa-B pathway. Under physiological conditions, p-RIPK1 inhibits RIPK3-dependent necroptosis (through FADD-mediated CASP8 recruitment to cleave RIPK1) and aberrant apoptosis during embryogenesis (by blocking TRADD-FADD interaction). It also drives inflammatory responses by enhancing pro-inflammatory cytokine (e.g., IL6) production. By similarity, it reciprocally phosphorylates RIPK3 and phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner to activate the MAP3K5-JNK apoptotic cascade.
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag C-6*His
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Accession
Q60855 (M1-S656)
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Gene ID19766
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Molecular Construction
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N-term
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RIPK1 (M1-S656)
Accession # Q60855 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
RIPK1; Receptor-Interacting Protein Kinase 1; Receptor Interacting Serine/Threonine Kinase 1; Receptor-Interacting Protein 1; RIP-1; Cell Death Protein RIP; RIP1; Serine/Threonine-Protein Kinase RIP; RIP; AIEFL; Receptor (TNFRSF)-Interacting Serine-Threon
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AA Sequence
MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCYHRSHGFVILKKVYTGPNRAEYNEVLLEEGKMMHRLRHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMHVLKTQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLKPENILVDRDFHIKIADLGVASFKTWSKLTKEKDNKQKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKSDVYSFGIVLWAIFAKKEPYENVICTEQFVICIKSGNRPNVEEILEYCPREIISLMERCWQAIPEDRPTFLGIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPVLQRMFSLQHDCVPLPPSRSNSEQPGSLHSSQGLQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEKQTKPQPRQNEAYNREEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQLSWPATQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPYFSGPVADDLIKYTIFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTSLTDEHLNPIRENLGRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMREGTKGATVGKLAQALHQCCRIDLLNHLIRASQS
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Predicted Molecular Mass
81.8 kDa
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Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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