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Rnase 1 Protein, Human (HEK293, His)

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

The RNase 1 protein functions as an endonuclease capable of catalyzing RNA cleavage specific to the 3' side of pyrimidine nucleotides. This enzymatic activity extends to single- and double-stranded RNA, demonstrating its versatility in RNA substrate recognition and processing. Rnase 1 Protein, Human (HEK293, His) is the recombinant human-derived Rnase 1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Rnase 1 Protein, Human (HEK293, His)
  • Rnase 1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The RNase 1 protein functions as an endonuclease capable of catalyzing RNA cleavage specific to the 3' side of pyrimidine nucleotides. This enzymatic activity extends to single- and double-stranded RNA, demonstrating its versatility in RNA substrate recognition and processing. Rnase 1 Protein, Human (HEK293, His) is the recombinant human-derived Rnase 1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

RNase 1 protein is an endonuclease that plays a crucial role in catalyzing the cleavage of RNA molecules, specifically targeting the 3' side of pyrimidine nucleotides. This enzymatic activity is not limited to a particular RNA conformation, as RNase 1 is known to act on both single-stranded and double-stranded RNA. By facilitating the precise cleavage of RNA molecules, RNase 1 contributes to the regulation and turnover of RNA in cellular processes. Its ability to target pyrimidine-rich regions suggests a broad range of potential substrates, highlighting its importance in RNA metabolism and cellular homeostasis.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P07998 (K29-T156)

  • Gene ID
    6035  [NCBI]
  • Molecular Construction
    • N-term
    • Rnase 1 (K29-T156)
      Accession # P07998
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    RNASE1; HP-RNase; Prev. RNS1; RNase A; RAC1; RIB-1; Ribonuclease, RNase A Family, 1 (Pancreatic); RIB1; Ribonuclease Pancreatic; Pancreatic Ribonuclease; Ribonuclease 1; Ribonuclease A C1; RNase UpI-1; Ribonuclease A; Ribonuclease A Family Member 1, Pancr

  • AA Sequence

    KESRAKKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST

  • Predicted Molecular Mass

    15.6 kDa

  • Molecular Weight

    Approximately 17-33 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 10% glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Rnase 1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Rnase 1 Protein, Human (HEK293, His)
  • Rnase 1 Protein, Human (HEK293, His) Product Image