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Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc)

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Based on 1 Customer Validation

Semaphorin-3F/SEMA3F proteins may play a role in cell motility and adhesion, suggesting involvement in fundamental cellular processes related to movement and interactions. The precise mechanisms and context by which SEMA3F regulates cell motility and adhesion are areas of interest, highlighting its potential significance in cell dynamics. Semaphorin-3F/SEMA3F Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-3F/SEMA3F protein, expressed by HEK293 , with N-6*His, N-Myc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc)
  • Experimental Validation Results for Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc)
  • Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Semaphorin-3F/SEMA3F proteins may play a role in cell motility and adhesion, suggesting involvement in fundamental cellular processes related to movement and interactions. The precise mechanisms and context by which SEMA3F regulates cell motility and adhesion are areas of interest, highlighting its potential significance in cell dynamics. Semaphorin-3F/SEMA3F Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-3F/SEMA3F protein, expressed by HEK293 , with N-6*His, N-Myc labeled tag.

Background

The Semaphorin-3F/SEMA3F protein is suggested to potentially play a role in cell motility and cell adhesion, indicating its involvement in fundamental cellular processes related to cell movement and interaction. The precise mechanisms and contexts in which SEMA3F operates in regulating cell motility and adhesion remain areas of interest, highlighting its potential significance in cellular dynamics. The versatility of SEMA3F in these processes suggests a multifaceted role in influencing cellular behaviors, emphasizing the need for further exploration to elucidate its specific functions and molecular mechanisms.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human Neuropilin-2 Protein at 2 µg/mL (100µL/well) can bind biotinylated Human SEMA3F. The ED50 for this effect is < 0.6 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human Neuropilin-2 Protein at 2 µg/mL (100µL/well) can bind biotinylated Human SEMA3F. The ED50 for this effect is 0.5048 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-6*His;N-Myc
  • Accession

    Q13275 (S19-T785)

  • Gene ID
    6405  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-Myc
    • SEMA3F (S19-T785)
      Accession # Q13275
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SEMA3F; Semaphorin IV; Semaphorin 3F; Sema IV; SEMAK; Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, 3F; Sema4; Semaphorin III/F; Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F; Sema III/F; S

  • AA Sequence

    SFPTQDHLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILLKDEDHDRMYVGSKDYVLSLDLHDINREPLIIHWAASPQRIEECVLSGKDVNGECGNFVRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQAVRGRGSRATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEELYAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSAERNDDKLYFFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFLKARLVCSVPGEDGIETHFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRGSAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFTPSMKSTKDYPDEVINFMRSHPLMYQAVYPLQRRPLVVRTGAPYRLTTIAVDQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQELEELMLEEVEVFKDPAPVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYCAWDGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVAGSAAFLECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRALQLSDRGLYSCTATENNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPPGAGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPSPREAPGAPRSPEPQDQKKPRNRRHHPPDT

  • Molecular Weight

    Approximately 90-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human Neuropilin-2 Protein at 2 µg/mL (100µL/well) can bind biotinylated Human SEMA3F. The ED50 for this effect is 0.5048 μg/mL.

  • Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc)
  • Experimental Validation Results for Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc)
  • Semaphorin-3F/SEMA3F Protein, Human (HEK293, His-Myc) Product Image