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Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 and is critical for intercellular signaling.It regulates synaptic development, promoting inhibitory synapses via PLXNB1 and excitatory synapses via PLXNB2.Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His)
  • Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 and is critical for intercellular signaling.It regulates synaptic development, promoting inhibitory synapses via PLXNB1 and excitatory synapses via PLXNB2.Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Semaphorin-4A/SEMA4A protein serves as a cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1, playing a crucial role in cell-cell signaling. Its involvement in regulating glutamatergic and GABAergic synapse development underscores its significance in synaptic processes. SEMA4A promotes inhibitory synapse development in a PLXNB1-dependent manner and excitatory synapse development in a PLXNB2-dependent manner. Beyond synaptic functions, SEMA4A contributes to adaptive immunity by priming antigen-specific T-cells and promoting the differentiation of Th1 T-helper cells. Additionally, SEMA4A facilitates the phosphorylation of TIMD2 and inhibits angiogenesis. Its role in axon growth cone collapse and the inhibition of axonal extension further highlights its influence in providing local signals to specify territories inaccessible for growing axons. The protein interacts with PLXNB1, PLXNB2, PLXNB3, PLXND1, and TIMD2, showcasing its versatile involvement in diverse cellular processes and signaling pathways.

Verified Bioactivity

Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED50 for this effect is 0.2-0.8 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED50 for this effect is 0.6023 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q62178 (T33-H682)

  • Gene ID
    20351  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA4A (T33-H682)
      Accession # Q62178
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SEMA4A; Semaphorin-4A; Prev. SEMAB; FLJ12287; CORD10; Sema B; SemB; RP35; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, (Semaphorin) 4A; SEMB; Semaphorin 4A; Semaphorin-B

  • AA Sequence

    TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPH

  • Molecular Weight

    Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED50 for this effect is 0.6023 μg/mL.

  • Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His)
  • Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) Product Image