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Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His)

Customer Review

Based on 1 Customer Validation

Semaphorin-7A/SEMA7A proteins critically regulate integrin-mediated signaling, affecting cell migration and immune responses. It promotes focal adhesion complex formation, activates PTK2/FAK1, and leads to the phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-7A/SEMA7A, expressed by HEK293, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His)
  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Semaphorin-7A/SEMA7A proteins critically regulate integrin-mediated signaling, affecting cell migration and immune responses. It promotes focal adhesion complex formation, activates PTK2/FAK1, and leads to the phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-7A/SEMA7A, expressed by HEK293, with C-6*His labeled tag.

Background

The Semaphorin-7A/SEMA7A protein plays a crucial role in integrin-mediated signaling, contributing to the regulation of cell migration and immune responses. It facilitates the formation of focal adhesion complexes and activates the protein kinase PTK2/FAK1, leading to the subsequent phosphorylation of MAPK1 and MAPK3. Moreover, it enhances the production of pro-inflammatory cytokines by monocytes and macrophages. This protein also plays a significant role in modulating inflammation and T-cell-mediated immune responses. Additionally, it promotes axon growth in the embryonic olfactory bulb and aids in the attachment, spreading, and dendrite outgrowth in melanocytes. It interacts with PLXNC1 and the integrins ITGA1 and ITGB1.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. When 5×104 cells/well are added to coated plates, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. The ED50 for this effect is 0.2644 μg/mL, corresponding to a specific activity is 3.782×103 U/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. When 5×104 cells/well are added to coated plates, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. The ED50 for this effect is 0.2644 μg/mL, corresponding to a specific activity is 3.782×103 U/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9QUR8 (Q45-A646)

  • Gene ID
    20361
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SEMA7A; CDw108; Prev. SEMAL; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, (Semaphorin) 7A; CD108; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, 7A; Semaphorin-7A; Semaphorin 7A (John Milton Hagen Blood Group); Sema

  • AA Sequence

    QGHSRSGPRISAVWKGQDHVDFSQPEPHTVLFHEPGSFSVWVGGRGKVYHFNFPEGKNASVRTVNIGSTKGSCQDKQDCGNYITLLERRGNGLLVCGTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLKGYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGETFHVLYLTTDRGTIHKVVESGDQDHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSQWEVSQVPLDMCEVYSGGCHGCLMSRDPYCGWDQDRCVSIYSSQRSVLQSINPAEPHRECPNPKPDEAPLQKVSLARNSRYYLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLREAQHWELLPEDRALAEQLMGHARALA

  • Predicted Molecular Mass

    68.4 kDa

  • Molecular Weight

    Approximately 75-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. When 5×104 cells/well are added to coated plates, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. The ED50 for this effect is 0.2644 μg/mL, corresponding to a specific activity is 3.782×103 U/mg.

  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His)
  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His) Product Image