Serpin A3 Protein, Human (K267R, HEK293, His)

Serpin A3 Proteinas, with unclear physiological function, inhibits neutrophil cathepsin G and mast cell chymase, involved in angiotensin-1 to angiotensin-2 conversion. Serpin A3 also interacts with DNAJC1. Serpin A3 Protein, Human (K267R, HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-6*His labeled tag and K267R mutation. The total length of Serpin A3 Protein, Human (K267R, HEK293, His) is 400 a.a., with molecular weight of 56-80 kDa.

While the physiological function of Serpin A3 remains unclear, it exhibits inhibitory activity against neutrophil cathepsin G and mast cell chymase. These enzymes play a role in the conversion of angiotensin-1 to the biologically active angiotensin-2. Additionally, Serpin A3 interacts with DNAJC1.

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH₂. The IC₅₀ is 15.152 nM, as measured under the described conditions.

Human

HEK293

C-6*His

P01011 (H24-A423, K267R)

12  [NCBI]

HPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELRYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

56-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.5 or pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 20 mM HEPES, 150 mM NaCl, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.