Copy Link Print Information

Serpin A3 Protein, Human (K267R, HEK293, His)

Customer Review

Based on 1 Customer Validation

Serpin A3 belongs to the serine protease inhibitor family and can interact with DNAJC1. Serpin A3 can inhibit neutrophil cathepsin G and mast cell chymase and participate in the conversion of ACE1 to ACE2. Serpin A3 Protein, Human (K267R, HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-6*His labeled tag and K267R mutation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin A3 Protein, Human (K267R, HEK293, His)
  • Serpin A3 Protein, Human (K267R, HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin A3 belongs to the serine protease inhibitor family and can interact with DNAJC1. Serpin A3 can inhibit neutrophil cathepsin G and mast cell chymase and participate in the conversion of ACE1 to ACE2. Serpin A3 Protein, Human (K267R, HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-6*His labeled tag and K267R mutation.

Background

Serpin A3, also known as alpha-1-antichymotrypsin (ACT or AACT), also known as cytostatic 24/25 protein, is a member of the serine protease inhibitor (serpin) family. Serpin A3 has been found to have inhibitory activity against neutrophil cathepsin G and mast cell tryptase, two enzymes involved in the conversion of angiotensin ACE1 to biologically active ACE2. In addition, Serpin A3 can interact with DNAJC1.

Verified Bioactivity

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 is 15.152 nM, as measured under the described conditions.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P01011 (H24-A423, K267R)

  • Gene ID
    12  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin A3 (H24-A423, K267R)
      Accession # P01011
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINA3; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3, Isoform CRA_a; Prev. AACT; Growth-Inhibiting Protein 24; ACT; Growth-Inhibiting Protein 25; Alpha-1-Antichymotrypsin; Alpha-1 Antichymotrypsin; Serpin Peptidase

  • AA Sequence

    HPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELRYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

  • Molecular Weight

    Approximately 50-80 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 20 mM HEPES, 150 mM NaCl, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Serpin A3 Protein, Human (K267R, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin A3 Protein, Human (K267R, HEK293, His)
  • Serpin A3 Protein, Human (K267R, HEK293, His) Product Image