1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Serpin B5/Maspin
  6. Serpin B5/Maspin Protein, Human (His)

Serpin B5/Maspin Protein, Human (His)

Cat. No.: HY-P7385A
COA Handling Instructions

Serpin B5/Maspin Protein is a tumor suppressor and a unique member of the serpin family. Maspin inhibits cancer cell invasion, attachment to the extracellular matrix, increases sensitivity to apoptosis, and inhibits angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and critically involved in mammary gland development. Maspin acts as an endogenous inhibitor of histone deacetylase (HDAC1). Serpin B5/Maspin Protein, Human (His) is the recombinant human-derived Serpin B5/Maspin protein, expressed by E. coli, with His labeled tag. The total length of Serpin B5/Maspin Protein, Human (His) is 375 a.a., with molecular weight of approximately 40-45 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $85 In-stock
50 μg $235 In-stock
100 μg $400 In-stock
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Description

Serpin B5/Maspin Protein is a tumor suppressor and a unique member of the serpin family. Maspin inhibits cancer cell invasion, attachment to the extracellular matrix, increases sensitivity to apoptosis, and inhibits angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and critically involved in mammary gland development. Maspin acts as an endogenous inhibitor of histone deacetylase (HDAC1). Serpin B5/Maspin Protein, Human (His) is the recombinant human-derived Serpin B5/Maspin protein, expressed by E. coli, with His labeled tag. The total length of Serpin B5/Maspin Protein, Human (His) is 375 a.a., with molecular weight of approximately 40-45 kDa.

Background

Maspin is a unique member of the serpin family that shares extensive homology with monocyte-neutrophil elastase inhibitor, PAI-2 and other serpins. Among the established anti-tumor effects of Maspin are the inhibition of cancer cell invasion, attachment to extracellular matrices, increased sensitivity to apoptosis, and inhibition of angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and angiogenesis and critically involved in mammary gland development. Maspin is an endogenous inhibitor of histone deacetylase (HDAC1) and reprograms tumor cells toward better differentiated phenotypes by inhibiting HDAC1.It stimulates an antitumor immune response in prostate tumor cells , leading to tumor elimination in vivo. Maspin may function as a modulator of the immune system in the tumor microenvironment[1][2].

Species

Human

Source

E. coli

Tag

His

Accession

P36952-1 (M1-P375)

Gene ID

5268

Synonyms
rHuMaspin; Serpin B5; PI-5
Molecular Weight

approximately 40-45 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS

Endotoxin Level

<1 EU/mL, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Serpin B5/Maspin Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Serpin B5/Maspin Protein, Human (His)
Cat. No.:
HY-P7385A
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