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Serpin B5/Maspin Protein, Human (His)

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Serpin B5/Maspin Protein is a tumor suppressor and a unique member of the serpin family. Maspin inhibits cancer cell invasion, attachment to the extracellular matrix, increases sensitivity to apoptosis, and inhibits angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and critically involved in mammary gland development. Maspin acts as an endogenous inhibitor of histone deacetylase (HDAC1). Serpin B5/Maspin Protein, Human (His) is the recombinant human-derived Serpin B5/Maspin protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin B5/Maspin Protein, Human (His)
  • Experimental Validation Results for Serpin B5/Maspin Protein, Human (His)
  • Serpin B5/Maspin Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Serpin B5/Maspin Protein is a tumor suppressor and a unique member of the serpin family. Maspin inhibits cancer cell invasion, attachment to the extracellular matrix, increases sensitivity to apoptosis, and inhibits angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and critically involved in mammary gland development. Maspin acts as an endogenous inhibitor of histone deacetylase (HDAC1). Serpin B5/Maspin Protein, Human (His) is the recombinant human-derived Serpin B5/Maspin protein, expressed by E. coli , with C-6*His labeled tag.

Background

Maspin is a unique member of the serpin family that shares extensive homology with monocyte-neutrophil elastase inhibitor, PAI-2 and other serpins. Among the established anti-tumor effects of Maspin are the inhibition of cancer cell invasion, attachment to extracellular matrices, increased sensitivity to apoptosis, and inhibition of angiogenesis. Maspin interacting with diverse group of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis, and angiogenesis and critically involved in mammary gland development. Maspin is an endogenous inhibitor of histone deacetylase (HDAC1) and reprograms tumor cells toward better differentiated phenotypes by inhibiting HDAC1.It stimulates an antitumor immune response in prostate tumor cells , leading to tumor elimination in vivo. Maspin may function as a modulator of the immune system in the tumor microenvironment[1][2].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human Maspin at 2μg/mL (100 μL/Well) can bind Anti- Maspin Antibody. The ED50 for this effect is 0.1551 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human Maspin at 2 μg/mL (100 μL/Well) can bind Anti-Maspin Antibody. The ED50 for this effect is 0.1551 μg/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P36952-1 (M1-P375)

  • Gene ID
    5268
  • Molecular Construction
    • N-term
    • Serpin B5 (M1-P375)
      Accession # P36952-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    SERPINB5; Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5; Prev. PI5; Peptidase Inhibitor 5; Maspin; Serpin B5; Serine (Or Cysteine) Proteinase Inhibitor, Clade B (Ovalbumin), Member 5; PI-5; Serpin Family B Member 5; Protease Inhibitor 5 (Maspi

  • AA Sequence

    MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP

  • Molecular Weight

    Approximately 43 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/mL, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human Maspin at 2 μg/mL (100 μL/Well) can bind Anti-Maspin Antibody. The ED50 for this effect is 0.1551 μg/mL.

  • Serpin B5/Maspin Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for Serpin B5/Maspin Protein, Human (His)
  • Experimental Validation Results for Serpin B5/Maspin Protein, Human (His)
  • Serpin B5/Maspin Protein, Human (His) Product Image